不同铂类对CNE-2细胞株放射增敏的体外比较实验分析.pdfVIP

irradiation with medical linear accelarator in 6MV X ray. Each group were given 0、0.5、1、2 、3、4 、6、8、10Gy of irradiation dose.The dose rate was 412.31cGy/min, SSD=100cm, PDD=97.232%,the irradiation area was 30 ×30cm. Fresh medium was replaced in four hours after irradiation, the CNE-2 cells cultured in the incubator at 37 ° C, 0.5% CO2 thermostat for 14d, records the killing effect of different treatments, calculate the survival rate, clone formation rate with application of counting colony-forming method;the mathematical model of the single-hit multi-target was used to plot survival curve (the GraphPad Prism5.0 software). Calculate N, D0, Dq, SERD0, SERDq, SERSF2, compare the difference of irradiation alone group and drug + radiation group, as well as different drugs + irradiation subgroup. Results ：The IC10 of Cisplatin, Carboplatin,Oxaliplatin and Nedaplatin on CNE-2 were 0.28ug/ml and 1.201527ug/ml 1.43ug/ml and 6.301118ug/ml respectively, the IC50 were 18.78ug/ml and 1.56808ug/ml respectively. The plating efficiency of CNE-2 was 72%. The value of N, D0 and Dq of CNE-2 cells received treatment of radiation alone were 2.305, 1.478Gy，1.234Gy respectively; the value of N, D , Dq SER 、SER and 0 D0 Dq SERSF2 of CNE-2 cells received Cisplatin+ radiation were 2.547, 1.011Gy， 0.945Gy, 1.462, 1.678, 1.579 respectively; those of CNE-2 cells received Carboplatin+radiation were 2.263, 0.994Gy, 0.812Gy, 1.487, 1.521,1.796 respectively; those of CNE-2 cells received Oxaliplatin+ radiation were 1.306, 0.969Gy, 0.789Gy, 1.524, 1.563, 1.884 respectively; Nedaplatin+radiation were 2.0