e1a基因增加鼻咽癌细胞放射敏感性的研究肿瘤学专业论文.docxVIP

硕士学位论文 硕士学位论文 中文摘要 细胞照射后细胞存活无明显差异，而E1A转染后的CNE．2细胞照射 后细胞存活明显下降，存活曲线的肩区变窄，Dq值降低。(3)4GY照 射后不同时间转染前后的细胞存活率以12小时最低，未转染的 CNE．2细胞与转染E1A的CNE．2细胞12小时存活率分别为：51．3％、 20．4％，经t检验均有显著差异。(4)4细胞周期出现G2／M期阻滞， RT-PCR结果显示E1A基因能提高wt．p53的表达。 2．裸鼠致瘤实验 结果显示：CNE2．Ad—E1A细胞形成的肿瘤较CNE2和CNE2．Ad．13 ．gal的出瘤时间晚，生长慢，瘤重小，抑瘤率为70．57％。模拟E1A 基因治疗临床应用的裸鼠实验结果显示：Ad．E1A、放疗及Ad．ElA+ 放疗3种治疗方法均可抑制肿瘤生长，与Ad．B．gal对照组相比，抑 瘤率平均值分别为：60．32％、70．53％和97．15％，与放疗组相比，E1A 基因加放疗组的抑瘤率为89．3％。RT．PCR显示E1A基因能提高鼻 咽癌组织wt．p53的表达。 结论： 1本实验的结果证实E1A使CNE．2细胞照射后的问期死亡和增 殖死亡均提高，使细胞的放射敏感性增加。上述作用可能与E1A基 因提高wt—p53基因的表达和引起G2／M期阻滞有关。 2．E1A基因能够明显抑制人鼻咽癌细胞裸鼠移植瘤的生长并显 著增加其对放疗的敏感性，具有较好的临床应用前景，该作用可能与 E1A基因提高鼻咽癌组织wt—p53的表达有关。 关键词：E1A基因，放射增敏，鼻咽癌细胞，wt．p53基因，放 射治疗 硕士学位论文 硕士学位论文 英文摘要 Abstract objective：To test ifElA gene is able to increase radiosensitivity of Nasopharyngeal Carcinoma cells and its mechanism．And to study the effects of E 1 A gene on the growth and Radiosensitivity of transplantation tumor of nude mice of human Nasopharygeal Carcinoma cell Methods：Ad-E 1 A was transfected into human Nasopharyngeal Carcinom cells(CNE2)，then the transfected cells were selected using G4 1 8，the positive colones were identified by RT-PCR．CNE2 cells，and cells transfected with CNE2··13·-gal and transfected with CNE2-·Ad·-E 1 A were irradiated with 0GY、1 GY、2GY、4GY、6GY、8GYrespectively using 6MV Xray．Above three cell lines were also given 4GY irradiation respectively survival ratios of these cells were analyzed timely with MTT method．The redistributions of cell cycle were analyzed by flow cytometry．RT-PCR was used to detect the expression of wt-p53．CNE2、 CNE2··Ad-13-gal and CNE2--Ad-E 1 A cells were transplanted into nude mice，then the time of tumor formation，growth rates and weight of tumor were observed．To study the effects of