健脑通络口服液（汤）对急性脑梗死脑保护的机制研究及临床疗效观察-中医内科学专业论文.docx

湖南中医1i5太学博士学位论文结论： 湖南中医1i5太学博士学位论文 结论： 1．缺血性中风病治疗以益肾补气以健脑、活血化瘀以通络，标本同治 为宜，其急性期亦不避补肾益精。 2该老龄大鼠经阻塞大脑中动脉造成的持续脑缺血，形成的脑梗死模 型是成功的。 3六味地黄汤和补阳还五汤均能改善急性持续脑缺血大鼠的 脑梗死体积、降低神经功能评分，调节缺血脑组织中GDNF、TGF—D l、 TNF．Ⅸ表达，保护神经元，促进缺血脑组织的修复，二者之间存在协同 效应；其中单用补阳还五汤优于单用六味地黄汤。 4健脑通络口服液能改善中医证候及疗效，其作用优于对照组消栓口服 液，尤其体现在显效率和改善半身不遂、言语謇涩、腰酸等症状方面。两组 总疗效相-3，无明显差异。 5健脑通络口服液能改善中医症状、改善神经功能缺损、提高日常生活 能力、改善急性脑梗死患者的临床疗效，其作用优于消栓口服液。 关键词：健脑通络口服液六味地黄汤补阳还五汤脑梗死脑缺血 模型协同效应 补肾益气活血法GDNF TGF—p l TNF．d脑保护 IV 湖南中区焉土学博士学位论文Brain—Protective 湖南中区焉土学博士学位论文 Brain—Protective Mechanism Study and Clinical Effects on Acute Cerebral Infarction by JNTLOL Doctor Graduate：Li guo-jing Director：Wang xing-kuan ABSTRACT Objective：To explore the Tradition Chinese Medicine’S(TCM’S)theory of treating ischemia stroke by the way of kidney．And study the effects on acute cerebral infarction by Jian Nan Tong Luo oral liquid(JNTLOL)via experimental and clinical aspect，as well as the cooperative effects of Liu Wei Di Huang Tang(LWDHT)and Bu Yang Huan Wu Tang(BYHWT)． Methods： 1．Explaining the relationship between kidney and brain，relationship between kidney and ischemia stroke，and explaining stroke’S cause and pathogenesis by utilizing the theory of brain’S disease of TCM，SO as tO supply new method for treating ischemia stroke． 2．Expermimental StUay：This study Was build on old·aged rat models of permanent cerebral ischemia made by middle cerebral artery occlusion(MCAO)， to detect it through the following experimental items：neurological impairment， volume of cerebral infarction,HE stain．imlnHllohistochemistry and in—situ hybridization at 24 hours．72 hours and 7 day after operation,to adopt wim 2×2 factorial design．so as to explore LWDHT and BY}nvT’S function of relieving neurological impairment and volume of cerebral infarction，regulating the expression of GDNF、TGF-p 1、TNF—a in ischemic tissue of brain，and the two prescripdon’S cooperative effects Was studied too． 3．Clinical effects Study：A single-blind controlled clinical expedment Was carried out．and