大鼠薄型子宫内膜模型的建立和鉴定.PDFVIP

15 卷 5 期 生命科学研究 灾燥造援员5 晕燥援5 圆园11 年 10 月 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Oct. 圆园11 大鼠薄型子宫内膜模型的建立和鉴定 红, 赵 静, 李艳萍* (中南大学 湘雅医院妇产科 生殖医学中心, 中国湖南 长沙410008) 摘 要: 为探索宫腔注入无水乙醇建立薄型子宫内膜模型的可行性, 将25 只大鼠分3 组: 对照组(宫腔注入生 理盐水), 5 分钟组(宫腔注入无水乙醇并贮留5 min), 10 分钟组(宫腔注入无水乙醇并贮留10 min), 经测量子宫 内膜变薄者为造模组, 5 分钟组有14 个子宫内膜变薄, 10 分钟组无一例纳入造模组. 免疫组织化学检测角蛋 白 波形蛋白 血管内皮生长因子(vascular endothelial growth factor, VEGF) 雌激素受体琢(estrogen related recep tor alpha, ER ) 的表达. 结果发现5 分钟组造模组与对照组单位内膜面积中角蛋白面积 单位间质面积中波形 蛋白面积和子宫内膜中VEGF 平均光密度差异有统计学意义 (P0.05), 造模组与对照组子宫内膜ER 组织学 积分无差异(P0.05). 表明宫腔注入无水乙醇并贮留5 min 可以成功建立薄型子宫内膜模型, 成功率为70%. 关键词: 薄型子宫内膜; 无水乙醇; 大鼠模型; 再生长受限 中图分类号: R71 文献标识码: A 文章编号: 1007 7847(20 11)05 0426 06 Establishment and Identification of Rat Thin Endometrium Model GAO Hong, ZHAO Jing, LI Yan ping* (Reproductive Medical Center, X iangya Hosp ital, Central South Unive rs ity, Changsha 410008, H unan, China) Abstract: To explore a novel method to establish a thin endometrium by injecting ethanol into the uterine cavity of rat. Twenty five female SD rats were divided into three groups: control group (physiological saline were injected into cavities and kept for 5 min), 5 minutes group (95% ethanol was injected and kept for 5 min) and 10 minutes group (95% ethanol was injected and kept for 10 min). The endometria which were thin ner than that of the control group would be taken as a new group model group, the others were control group. Observe endometrial morphology by hematoxylin eosin staining (HE) and analyze the growth of the epithelial