壳聚糖载体耦联骨形态发生蛋白2目的基因转染-中国组织工程研究.PDF

中国组织工程研究 第 16 卷 第34 期 2012 –08 –19 出版 Chinese Journal of Tissue Engineering Research August 19, 2012 Vol.16, No.34 壳聚糖载体耦联骨形态发生蛋白2 目的基因转染MC3T3-E1细胞后 细胞生物学变化**★ 赵 刚，高 雪，莫宏兵，平玉卓，刘可俗 Biological changes of mesenchymal cells 3T3-E1 transfected by chitosan coupled bone morphogenetic protein-2 target gene Zhao Gang, Gao Xue, Mo Hong-bing, Ping Yu-zhuo, Liu Ke-su 文章亮点： 利用壳聚糖纳米粒载体耦联生物大分子骨形态发生蛋白2 转染MC3T3-E1 细胞，促进MC3T3-E1 细胞的增殖与分 Stomatology Medical 化，实现了骨诱导蛋白的局部持续表达，可加速颌骨的愈合。 College of Jiamusi University, Jiamusi 154002, Heilongjiang Abstract Province, China BACKGROUND: It has become one of the most promising fields for the application of growth factor in gene treatment . Zhao Gang★, Bone morphogenetic protein (BMP) can induce mesenchymal cells into osteoblasts and new bone formation, which plays Master, Associate professor, an important role in bone healing. Stomatology Medical College of Jiamusi OBJECTIVE: To realize the continuous expression of BMP partly and to promote cell proliferation and differentiation University, Jiamusi through transfecting mesenchymal 3T3-E1 cells (MC3T3-E1) in vitro with BMP-