响应曲面法优化微波辅助提取平菇多糖工艺研究-中国科技论文在线.PDF

中国科技论文在线 68 2010, Vol. 31, No. 04 食品科学 ※工艺技术 响应曲面法优化微波辅助提取平菇多糖 工艺研究 朱彩平，高贵田，李建科，赵艳红，邓典典 (陕西师范大学食品工程与营养科学学院，陕西西安 7 10062) 摘 要：为优化平菇多糖的微波辅助提取工艺，在单因素试验的基础上，选择提取时间、微波处理功率以及液料 比为自变量，多糖得率为响应值，应用Design Exp ert 7. 1.6 软件技术，采用响应曲面法设计、分析研究各自变量 及其交互作用对多糖得率的影响。利用响应面分析方法，模拟得到二次多项式回归方程的预测模型，并确定平菇 多糖微波辅助提取工艺的最佳条件为提取时间10min 、微波处理功率420W 、液料比40 :1(mL/g) 。在此条件下，多 糖得率达到9 .04 % 。 关键词：平菇多糖；微波辅助提取；响应面分析 Response Surface Analysis for Optimizing Microwave-assisted Extraction of Pleurotus ostreatus Polysaccharide ZHU Cai-ping ，GAO Gui-tian ，LI Jian-ke ，ZHA O Yan-hong ，DEN G Dian-dian , (College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi an 7 10062, China) Abstract ：To optimize the microwave-assisted extraction process of Pleurotus ostreatus polysaccharide (POP), single factor experiments involving three technological parameters including extraction time, microwave power and solvent/material ratio were done for following investigations on the interactive effects of the parameters on POP yield using central composite design (CCD) combined with response surface methodology. In the optimization, Design Expert 7.1.6 software was used to fit CCD experimental data and a quadratic polynomial regression model was developed for POP yield as a respon se to the above parameters. Results showed that the optimal values of technological parameters for using deionized water to extract POP were as follows: extraction time 10 min, microwave power 420 W and solvent/material ratio 40:1 (mL/g). Under such conditions, the yield of POP was up