超高效液相色谱快速测定饮料中的16种食品添加剂.PDF

※分析检测 食品科学 2010, Vol. 31, No. 02 195 超高效液相色谱快速测定饮料中的 16种食品添加剂 王 骏，胡 梅，张 卉，张喜琦，祝建华 ( 山东省产品质量监督检验研究院，国家加工食品质量监督检验中心( 山东) ，山东济南 250 100) 摘 要：建立快速测定饮料中16 种食品添加剂的分析方法。样品中的食品添加剂用甲醇- 水提取，在ACQUITY R UPLCO BEH C18 色谱柱上分离，用二极管阵列检测器检测。结果表明，16 种食品添加剂在5.5min 内可获得良好的 分离，各组分检出限均优于1.0mg/kg ，加标回收率为9 1.6%～100.3% ，RSD 为0.66%～2 .20% ，表明该方法精密度 良好。 关键词：超高效液相色谱；饮料；食品添加剂；测定 Rapid Determination of Sixteen Kinds of Food Additives in Beverages by Ultra Performance Liquid Chromatography WAN G Jun ，HU Mei ，ZHAN G Hu i ，ZHAN G X i-qi ，ZHU Jian -hu a (Shandong Supervision and Inspection Institute for Product Quality , National Centre for Quality Supervision and Testing of Processed Food (Shandong), Jinan 250 100, China) Abstract ：An ultra performance liquid chromatographic (UPLC) method was presented for the rapid determination of 16 kinds of food additives in beverages. The method was based on extraction with methanol mixed with water, separation on an R ACQUITY UPLC O BEH C18 column and detection with diode-array detector. All the sixteen kinds of food additives were well separated within 5.5 minutes. The limits of detection for them were more than 1.0 mg/kg, and the recoveries at three spike levels were 9 1.6% －100.3%, RSD was 0.66% —2.20%. Key words ：ultra performance liquid chromatography (UPLC)；beverage ；food additives ；determination 中图分类号：TS202.3 ；O657.72 文献标识码：A 文章编号：1002-6630(20 10)02-0 195-04 随着食品工业的快速发展，食品添加剂品种不断增 定方法，可用于各类饮料中食品添加剂的快速筛查，为 加。目前全世界应用的食品添加剂品种已多达25000 余 食品添加剂的安全使用和监管提供技术支撑。 种，我国2007 年发布的GB 2760 《食品添加剂使