散发性结直肠癌的MSI与临床病理特点的关系.docxVIP

散发性结直肠癌的MSI与临床病理特点的关系(成都中医药大学附属医院四川成都610072)【摘要】目的：通过微卫星位点的分析，了解散发性结直肠癌中的微卫星不稳定性的阳性率，并探讨其与临床病理特点的关系。方法：收集131例结直肠癌的血液和肿瘤标木，分离DNA,荧光标记引物后通过PCR扩增，并在DNA 测序仪上进行毛细管电泳，Genescan软件收集扩增片段进行分析。结果：131例结直肠癌中27例(20.6%)为MSI?H阳性,30例(22.9%)为MSI?L, 75例(57.3%) 为MSS。MSI与患者的年龄，肿瘤的大小相关(Plt;0.05),而在性别、发病部位、肿瘤分化程度方面差异无统计学差异(Pgt；0.05)o结论：根据MSI对散发性大肠癌进行分类有重要的理论和实际意义。【关键词】散发性结直肠癌；微卫星不稳定；病理特点【中图分类号】R730.2【文献标识码】A【文章编号】1007-8231 (2016) 22-0025-02Microsatellite instability of sporadic colorectal cancer and its clinicopathological sign 讦icanceThe Affiliated Hospital of Chengdu University of Traditional ChineseMedicine,Chengdu 610072,Sichuan’China[Abstract] Objective To investigate the incidence of microsatelliteinstability(MSI) in sporadic colorectal cancer and the association with clinicopathological character. Methods The blood and tumor samples of 131 cases of colorectal cancer were collected. Genomic DNA was extracted from tumor tissues and matched blood. Microsatellite an alysis was performed using fluoresce nt PCR followed by fragment analysis on automatic DNA sequencer with Genescan software.Results 27 out of 131 samples was MSI-H and significantly associated with patient age and tumor size(Plt;0.05),but not tumor site, stage nor differentiation(Pgt;0.05). Conclusion The classification of sporadic CRC according to MSI may be of im porta nee both theoretically and practically.[Key words] Sporadic colorectal can cer; Microsatellite in stability;Clinicopathological feature大肠癌是最常见的恶性肿瘤之一，其发生、发展是一个多基因多步骤的复杂过程，其中涉及到癌基因的激活和抑癌基因的失活。基因遗传不稳定性如微卫星不稳定(microsatellite instability, MSI)可能是产生突变，导致肿瘤发生、发展的重要分子事件。本研究就最近收治的散发性大肠癌的MSI水平进行检测, 并探讨其临床病理生物学特征。1 .资料与方法1.1病例收集收集成都中能够医药大学附属医院病理科2015年?2016年诊断的散发性结直肠癌131例，术前均未经过化疗，其中男性68例，女性63例，年龄27?92岁。病变部位分为近端结肠39例，远端结肠21例，直肠71例。肿瘤术后病理类型：HE染色，3例缺失，有11例为黏液腺癌，2例低分化腺癌，其余口5 例为管状腺癌。高分化5例，中分化114例，低分化11例。1.2 DNA的提取采用TIANGEN的血液/细胞组织基因组DNA提取试剂盒(目录号DP304-03),严格安装说明书提取血液和肿瘤组织DNAo提取后在Nanodrop 2000 微量紫外分光光度计上进行浓度及波峰测试，并进行DNA电泳。1.3微卫星位点的分析1.3.1聚合酶链反应(PCR)： BAT-25, BAT-26引物序列见表1,由