固定化复合芽孢杆菌微生态制剂产品研制-生物工程专业论文.docxVIP

固定化复合芽孢杆菌微生态制剂产品研制The 固定化复合芽孢杆菌微生态制剂产品研制 The Development of Immobilized Composite Probiotics Products of Bacillus Abstract With the intensive development pattern of mariculture industry becoming more and more widespread，water pollution problem is increasingly serious，in which ammonia nitrogen level has been the major restrictive factor．Microecologics has been widely applied to improve water quality in aquaculture．Probiotics has two main functions，namely，improving water quality in aquaculture ponds and antagonizing pathogens．Studies have shown that the Bacillus Can improve water quality by reducing the ammonia nitrogen and nitrate content effectively． Besides，as a kind of probiotic bacteria，bacillus can inhibit pathogenic bacteria to improve the water ecological environment．This study aims to develop immobilized composite bacillus microecologics suitable for mariculture，in order to solve the problem of high ammonia nitrogen content in mariculture． Ammonia nitrogen removing Bacillus has been screened and compared with the Bacillus from ACCC in ammonia nitrogen degradation rate，ammonia oxidase(amoA)and lactonase (AiiA)gene PCR under the same experimental conditions．The experimental results showed that the ammonia nitrogen degradation rate of A4 strain is the highest which reached 73．54％ with the inoculum dosage of 2％．decreasing the ammonia nitrogen value from 25 mg／L to 5．73 mg／L．AiiA lactonase gene has been amplified and sequenced from A1 strain by PCR． Preliminarily determines the A 1 strain carded within the AiiA esterase gene．have certain which preliminarily determines antibacterial potential of A1 by the degradation of quorum sensing signal molecules AHL produced by pathogenic bacteria．The AHL degradation function of AiiA lactone enzyme in AI has been verified quantitatively by method of ONPG reaction with the use of AHL indicating bacteria JZA 1． The effect of ammonia nitrogen degradation of A4 strain immobilized by different carriers and the influence of superfi