碱浸提和纤维素酶辅助提取蓝莓多糖及其抗氧化能力的对比研究.pdfVIP

94 粮食与油脂 2017 年第 30 卷第 4 期 碱浸提和纤维素酶辅助提取蓝莓多糖及其 抗氧化能力的对比研究 王　鹏，郭　丽，孔　璐，关海宁 （绥化学院食品与制药工程学院，　黑龙江绥化　152061 ） 摘　要：以蓝莓为原料，利用碱浸提法和纤维素酶辅助法提取蓝莓多糖。采用正交试验优化蓝莓 多糖提取工艺，确定蓝莓多糖得率影响的主次顺序，碱浸提法为提取温度、碱添加量、提取次数、提 取时间，纤维素酶辅助法为pH 、纤维素酶添加量、提取时间、提取温度，纤维素酶辅助法比碱浸提 法获得的多糖得率提高了26%。2 种方法获得的蓝莓多糖均具有抗氧化活性，碱浸提蓝莓多糖清 除羟自由基能力最强，为88.8%，纤维素酶提取蓝莓多糖清除超氧阴离子自由基和羟自由基能力较 强，分别为76.2% 和76.0%。 关键词：蓝莓多糖；碱浸提；纤维素酶；抗氧化活性 Comparison of antioxidant capacity of blueberry polysaccharide by alkali extraction and cellulase assisted treatment WANG Peng ， GUO Li ，KONG Lu ，GUAN Hai-ning （College of Food and Pharmaceutical Engineering ，Suihua University ，Suihua 152061 ， Heilongjiang ，China ） Abstract ：With blueberry as raw material ，the blueberry polysaccharides were extracted by alkali extraction and cellulase assisted treatment. The extraction technology of blueberry polysaccharides was optimized by orthogonal experiment method to determine primary and secondary influence order to the yield of polysaccharide. The results showed that the order was extraction temperature ，amount of alkali ， extraction times and treatment time with alkali extraction ，and pH ，amount of cellulose ，treatment time and extraction temperature with cellulase assisted treatment. The yield of polysaccharides with cellulase assisted treatment increased by 26% than that with alkali extraction. The blueberry polysaccharides had high antioxidant activity by two extraction procedures ，by alkali extraction ，the scavenging capacity of hydroxyl radical was 88.8%，by cellulase assisted treatment ，radical scavenging capacity of superoxide anion and hydroxyl radical were 76.2% and 76.0%. Key words ：blueberry polysaccharide ；alkali extraction ；cellula