非毒性结节性甲状腺肿蛋白质表达差异初步探究.docxVIP

非毒性结节性甲状腺肿蛋白质表达差异初步探究 ［摘要］目的比较非毒性结节性甲状腺(NTNG)组 织和正常甲状腺组织整体蛋白质表达，以研究非毒性结节性 甲状腺组织蛋白质表达差异。方法提取NTNG甲状腺组织 和正常甲状腺组织总蛋白，利用双向凝胶电泳技术分离各组 总蛋白；经MALDI- TOF / TOF质谱分析，搜索数据库，寻 找匹配的相关蛋白质，鉴定蛋白质，获得差异蛋白质的信息。 结果经过初步筛选和质谱分析共获得9个具有统计学意义 的表达差异蛋白点，其中4个在非毒性结节性甲状腺高表达, 5个低表达。结论NTNG与正常腺体组织间存在多种差异表 达的蛋白质，可能通过细胞外基质、细胞因子、受体信号转 细胞信号传递等参与NTNG的发生和发展。 ［关键词］蛋白质组学；非毒性结节性甲状腺肿；蛋白 质表达 ［中图分类号］R581 ［文献标识码］A ［文章编号］ 2095-0616 (2013) 24-23-03 Primary study on the difference of protein expression of nontoxic nodular goiter YAO Jinke HU Caixue WU Genggang HE Wenguang Department of General Surgery , Zengcheng People， s Hospital, Zengcheng 511300, China [Abstract] Objective To analyze the comparison of overall protein expression between nontoxic nodular goiter (NTNG) tissues and normal thyroid tissues, and to explore the difference of protein expression of NTNG. Methods Total proteins of NTNG tissues and normal thyroid tissues were extracted and separated via two-dimensional gel electrophoresis ； the proteins were analyzed via MALDI-TOF/TOF mass spectrum , relevant proteins were matched in searching of database and then identified, and finally the infonnation of different proteins were obtained? ResuIts Nine differentially expressed protein spots which were statistically significant were obtained via primary screening and analysis of mass spectrum, four of which showed high expression in NTNG tissues, while five of which showed low expression. Conclusion There are various types of differentially expressed proteins between NTNG and normal thyroid. Extracellular matrix, cytokines, trans-cellular transduction of receptor signals are likely to be involved in the onset and development of NTNG. _Key words] Proteomics； Nontoxic nodular goiter (NTNG)； Protein expression 非毒性结节性甲状腺肿(nontoxic nodular goiter, NTNG)是一种常见的甲状腺疾病，发病率可高达6%?7%。以 女性多见。与正常的甲状腺细胞相比，非毒性结节性甲状腺 肿的甲状腺细胞在蛋白质水平上应有差异。利用这方面的指 标的差异，研究非毒性结节性甲状腺肿表达存在差异的相关 蛋白质的变化，有助于阐明其发生发展的机制。现利用蛋白 质组技术，初步研究分析NTNG患者甲状腺组织及正常甲状 腺组织之间表达明显差异的蛋白质。 1材料与方法 1实验标本 所有标本均取自增城市人民医院2009年1