固化小球藻（chlorella+vulgaris）对水体中三丁基锡的吸收和生物降解-食品安全生物学专业论文.docxVIP

金靖 金靖 中山大学硕士学位论文摘要 3． 研究了主要的环境因素(温度、悬浮颗粒、同系物和菌类)对固化藻球 去除TBT能力的影响。实验设计了三种水温(10 4-2。C、22士2。C、37-4-2。C)， 三种水体浑浊度(5 ntu、65 ntu、130 ntu)，三种TBT同系物三苯基锡(TPhT) 水平(O pg Sn l～、 100 gg Sn 1一、200 gg Sn l。)，以及一组灭菌和不灭菌河水对 照组，观察各环境因子对固化小球藻清除100 lag Sn L’1TBT能力的影响。实验结 果显示：在小球藻清除水体环境TBT的过程中，适当提高环境温度，将有助于 提高TBT降解率；水体中的沙悬浮颗粒在本实验条件下对TBT的生物降解并无 影响；本实验条件下，水环境中TPhT的添加有助于TBT的生物降解；自然河 水中的菌类并不影响TBT的生物降解。 关键词： 三丁基锡，固化小球藻，吸收，生物降解 II 金靖 金靖 中山大学硕士学位论文摘要 Studies of biosorption and biodegradation of tributyltin by Dbilizedilize microalgae(Chlorellalorella vulgaris Maj or：Food Safety Biology Master’S Candidate：Jing Jin Supervisor：Asso．Prof．Tian—Gang Luan ABSTRACT Biosorption and biodegradation by microorganisms to remove tributyltin(TBT)， a toxic and persistent contaminant，have attracted more and more interests in recent years but information related to microalgae is still inadequate．The present study aims to investigate the removal and degradation of TBT at 1 0，50 and 1 00 lag Sn 1。1 contamination levels by alginate—immobilized Chlorella vulgaris beads during six consecutive cycles(four days each)，and effects of environmental factor such as nutrients level，temperature，suspended solids，TPhT(the homologous of TBT)， bacteria or fungi on TBT removal． 1．A experiment was undertaken to investigate TBT removal efficiency of alginate immobilized cells of Chlorella vulgaris，a commercial species with resistance to TBT during six consecutive TBT treatment cycles at three concentration(1 0、50、 l OOp．g Sn L_)，compare the performance with blank alginate beads(without algae)．． Alginate immobilized Chlorella vulgaris beads could effectively remove TBT(over 90％)at different contamination levels for at least six consecutive cycles．The initial removal mechanism was physico-chemical adsorption onto alginate matrix with some onto cell walls．The TBT was then entered the cells and degraded into DBT，MBT and even inorganic tin．Cell growth was maintained after immobilization and treated with TBT although repeated ex