含氟羟基磷灰石薄膜特性及其生物相容性-材料学专业论文.docxVIP

浙江上学硕士学往论文基磷灰石沉积层越来越均匀，沉积方式由岛状向层状转变，说明生物活性随之增 浙江上学硕士学往论文 基磷灰石沉积层越来越均匀，沉积方式由岛状向层状转变，说明生物活性随之增 加。FHA薄膜CPBS浸泡实验结果表明：随着薄膜中氟含量的增加，有更小的溶 解性。上述的实验结果进一步验证了FHA薄膜具有良好的生物活性和低溶解性， 从物理化学变化角度上来讲，FHA薄膜非常适合于用作金属种植体表面的高品质 生物活性薄膜。 通过MTT法，流式细胞仪测定细胞周期，以及细胞生长曲线的测定，分析研 究了FHA薄膜材料对成骨细胞增殖生长的影响以及材料的浸提液对细胞增殖的 影响，通过细胞相对增殖活性的测定对FHA薄膜进行毒性评级。FHA薄膜的毒 性级别为0级或1级，可以认为不表现毒性。进一步实验结果显示，氟的存在非 但没有影响到成骨细胞的贴壁增殖生长，反而一定程度上诱导骨细胞的贴壁生长。 使得成骨细胞表现出更好的增殖活性。 本工作中获得的FHA薄膜具有理想氟分布，良好的物理化学性能和良好的生 物相容性。因此，本论文工作结果无论是在理论上还是在实际应用中，都有一定 的指导意义。 关键词：溶胶一凝胶含氟羟基磷灰石薄膜生物活性细胞相容性 II 浙江大学硕士学位论文Abstract 浙江大学硕士学位论文 Abstract Hydroxyapatite(HA)films on Ti6A14V substrates has been used as a kind of hard tissue replacement．HA possesses well biocompatibility，which has been discovered through test and in clinical application．However，the relatively large dissolution or resorption rates of HA will result in the failure of the implants．Fluoridated hydroxyapatite(HFA)in whose structure F‘occupies partially the position of OH—in HA， is expected to be more resistant to body fluid and less soluble than HA and still possess almost the smne bioactivity as HA．HFA will be used as the substitute of HA to repair and replace hard tissues in dentis仃y and orthopaedics fields． Under the foundation of prophase research，the distributing of F in HFA films has been designed to be lower in the surface and higher in the part which contacts substrates，which enables films to have well biocompatibility,reduce their solubility and improve the bond intension with substrates．In this work．FHA films on Ti6A14V substrates，which contain deferent fluorine contents，were prepared by sol-gel dip—coating method using hexafluorophosphoric acid(I-IPF6)as a fluorine-introducing reagent． The films were characterized by diffraction，X-ray Photoelectron Spectroscopy， scanning electron microscopy,Secondary Ion Mass Spectrometer to research their HFA phase，morphology，fluorine content and fluorine distribution．Some conclusions will be described as follow： With the increase of fluorine c