乳腺癌淋巴细胞亚群与不同分子亚型及临床病理特征的意义研究.docVIP

乳腺癌淋巴细胞亚群与不同分子亚型及临床病理特征的意义研究 ［摘要］目的检测不同分子亚型乳腺癌患者外 周血淋巴细胞亚群的变化，探讨其与临床病理特征的 关系。方法选取广西医科大学附属肿瘤医院2013 年1?12月就诊收治的206例女性乳腺病患者，随机 将其分为166例乳腺癌组(观察组，n=166)及40例 正常健康组(对照组，n=40)，观察组采用免疫组化 Envision二步法检测雌激素受体(ER)、孕激素受体 (PR)、人表皮生长因子受体-2 (HER-2)、Ki-67的蛋 白表达情况并进行分子分型，后采用流式细胞仪法测 定两组的外周血各淋巴细胞亚群CD3+、CD4+、CD8+、 CD16+CD56+、CD19+的活性，结合临床和病理特征 资料进行比较分析。结果在Luminal A型、Luminal B型及HER-2过表达型中，CD3+活性、CD4+/CD8+ 比值与正常健康组比较均下降，差异有统计学意义(P ［关键词］乳腺癌；淋巴细胞亚群；分子分型；临 床病理特征 ［中图分类号］R737.9 ［文献标识码］A ［文章编号］ 1674-4721 (2016) 04 (b) -0008-04 [Abstract] Objective To detect the changes of peripheral blood lymphocyte subsets in patients with different subtypes of breast cancer，and explore the relationship between different subtypes of breast cancer and their clinical pathological characteristics. Methods A total of 206 patients with women breast disease were selected from January to December 2013 in Affiliated Tumor Hospital of Guangxi Medical University，and were randomly divided into for 166 cases of patients with breast cancer group (observation group，n = 166) and 40 cases of healthy people as normal healthy group (control group，n = 40) .Immunohistochemistry envision two-step was used to detect the expression of estrogen receptor (ER)，progesterone receptor (PR)， human epidermal growth factor receptor-2 (HER-2) and Ki-67，using flowcytometry to detect the two group the of peripheral blood lymphocyte subgroup of CD3+， CD4+，CD8+，CD16+CD56+，CD 19+ activity，combined with the clinical and pathological features were analyzed and compared. Results Compared with the normal healthy group，the percentage of CD3+ and CD4+/CD8+ was significantly decreased in the groups of Luminal A，Luminal B type and HER-2 over expression （P ［Key words］ Breast cancer； Lymphocyte subsets； Molecular subtype； Clinicopathology characteristics 乳腺癌是女性最常见的恶性肿瘤之一，好发于中 老年女性，近年来乳腺癌发病率和死亡率呈逐年上升 的趋势［1-2］。乳腺的发病率和死亡率受多种因素的影 响，如患者的年龄、种族、心理因素以及肿瘤的大小、 组织分级、临床分期等［3］。近年来，乳腺癌分子分型 的研究已成为临床研宄的热点，Perou等［4］提出的乳 腺癌分子分型指出乳腺癌个体间的临床特点及预后存 在差异。近年来关于细胞免疫与乳腺癌的发生、治疗 及预后之间关