产烃葡萄藻在高温环境的生长特征研究【毕业】.docVIP

本科毕业论文 （20 届） 产炷葡萄藻在高温环境的生长特征研究 摘 要：微藻生物燃料是近年来新型生物质能源领域的热点z—。葡萄藻因含油量高、 燃烧值高成为n前微藻燃料开发的百选藻种。葡萄藻的培养成本过高限制了葡萄藻住物燃料 的发展，因此开发成本低廉而高效的葡萄藻培养技术具有重要意义。本文依据烟道气尾气的 高温环境，研究了匍萄藻在30° C、35° C、40° C三种高温下的生长特性，以期为利用烟道 气培养葡萄藻提供理论依据。结果表明：葡萄藻在三种高温逆境下的牛长速率均受到抑制， 表现为藻液0D迦、叶绿素含量、胡萝卜含量、细胞或集落数冃等都会随着培养时间延长而迅 速下降，口温度越鬲，下降速率越明显。因此如果想利用烟道气尾气为葡萄藻培养提供碳源 需要开发能够适应高温的葡萄藻漠种。 关键词：微藻燃料；葡萄藻；高温；生长特性 Growth characteristics of Botryococcus braunii 765 underhigh temperature Abstract： With the rapid development of global economy, fossil energy is running out. Therefore, the development of new energy, especially, the development of renewable energy is one of the top and most important global issues. Microalgae grow fast and contain high rate of oil. Microalgal calorific value is high and it docsift produce much carbon dioxide? Thus, microalgae are a kind of promising biomass. However, the cost of biofuel production of microalgae is high, which prenvents the development of microalgal biofuel. For using flue gas as a carbon source to culvtivate Bottyococcus braunii, this study researched the effects of high temperature on B.braunii 765? The results showed: the growth of B.braunii 765 would be inhibited obviously when the temperature was higher than 30°C, and the higher the temperature, the more seriously of the inhibition. Therefore, new B.braunii species that could tolerate high temperature as 40°C should be discovered or induced? Key words: Botryococcus braunii; microalgal biofuel; high temperature; growth characteristics TOC \o 1-5 \h \z 1.1研究背景 3 1.2国内外研究现状 3 1.2.1能源微藻藻种选育 3 1.2.2葡萄藻培养可行性 3 1.3 彳 7 F 丿 l* 丿 Qi j 4 1.3.1研究思路 4 1.3.2技术路线 4 HYPERLINK \l bookmark8 \o Current Document \h 2材料与方法 4 2.1藻种來源与培养 4 2.2丿匕牛物）、/.醫构建J试验设计 5 2.3试验参数测定分析 5 2.3.1藻细胞集落数目测定 5 2.3.2藻液pH值和DO值测定 6 2.3.3藻细胞干重和藻液吸光值测定 6 234藻液叶绿素含量测定 6 2.3.5藻液胡萝卜素含量测定 6 2.3.6藻液NOs-N含量测定 6 2.3.7藻液TP含量测定 6 3结果与讨论 3.1 r^j Ahl对藻PH ?和 DO 的影响 7 3.2高温对葡萄藻生长速率和生物量累积的影响 7 3.3高温对葡萄藻色素含量的影响 8 3.4高温对葡萄藻细胞和集落形态的影响 9 3.5高温对葡萄藻氮磷利用效率的影响 10 ??????? 114结论与展望 ???