反复种植失败患者周围血树突状细胞数量和功能分析-中国免疫学杂志.PDF

曾摇 勇等摇 反复种植失败患者周围血树突状细胞数量和功能分析摇 第2期 ·239 · doi:10.3969/ j.issn.1000鄄484X.2016.02.021 反复种植失败患者周围血树突状细胞数量和功能分析淤 于 于 于 于 于 于 于 于 曾摇 勇 摇 黄春宇 摇 陈摇 现 摇 梁佩燕 摇 刁梁辉 摇 陈摇 聪 摇 张摇 谞 摇 尹摇 彪 (深圳中山泌尿外科医院生殖医学中心,中山生殖与遗传研究所,深圳 518045) 摇 摇 中图分类号摇 R318郾16摇 摇 文献标志码摇 A摇 摇 文章编号摇 1000鄄484X(2016)02鄄0239鄄05 [摘摇 要]摇 目的:了解反复种植失败(Repeated implantationfailure,RIF)患者周围血树突状细胞(Dendritic cells,DC)数量 和功能的变化。 方法:采集30例RIF患者及15例正常对照组的黄体中期周围血,采用流式细胞术(Flow cytometry,FCM)对其 - + DC细胞功能分子及亚群进行检测。结果:与正常对照组相比,RIF患者的lin HLA鄄DR DC细胞占白细胞的比例无显著性变化 (P0郾05),DC细胞表面的共刺激分子(CD80 和CD86)以及免疫耐受分子CD200也无显著性变化(P0郾05)。 然而,RIF患者 + - - + 中CD11c CD123 mDC细胞比例显著升高(P0郾05),而CD11c CD123 pDC细胞比例无显著性变化(P0郾05)。结论:RIF患 者的周围血mDC细胞比例显著升高,其可能是影响RIF患者妊娠结局的因素之一。 [关键词]摇 母胎免疫耐受;反复种植失败;周围血;树突状细胞 Analysis ofquantityandfunctionofdendriticcellsinperipheralbloodofpatients with repeated implantation failure ZENG Yong,HUANG Chun鄄Yu,CHEN Xian,LIANG Pei鄄Yan,DIAO Liang鄄Hui,CHEN Cong,ZHANG Xu,YIN Bi鄄 ao郾Fertility Center of ShenzhenZhongshan Urology Hospital,ShenzhenZhongshanInstituteforReproductiveMedicine and Genetics,Shenzhen518045,China [Abstract]摇 Objective:To evaluate the quantity and function changes of dendritic cells (DC) in peripheral blood of patients with repeated implantation failure (RIF)郾Methods:30 patientswith RIF and 15 normal controls were enrolled in this study,and the peripheral blood was collected during the mid鄄luteal phase郾The percentage of DC subsets and the expression levels of functional - + molecules were assessed by flow cytometric analysis郾Results:Comparedwith normal controls,thepercentageof lin HLA鄄DR D