桂牧一号象草体外消化特性的研究-动物营养与饲料科学专业论文.docxVIP

2．尼龙袋——静态发酵法研究桂牧一号象草养分消化率：利用尼龙袋法进 2．尼龙袋——静态发酵法研究桂牧一号象草养分消化率：利用尼龙袋法进 行静态发酵，研究桂牧一号象草干物质(DM)、粗蛋白、可溶性蛋白、真 蛋白、粗纤维、中性洗涤纤维、酸性洗涤纤维在瘤胃液中的降解规律。结 果表明：桂牧一号象草体外发酵12h、24h、48h干物质降解率分别为23．39％、 33．95％、45．74％；蛋白组中粗蛋白和真蛋白的降解率随消化时间的延长逐 步升高，可溶性粗蛋白质降解率在O．12h逐步上升，12h．24h降解率呈下降 趋势，呈S形分布，48h内蛋白质这种前期(0．12h)消化率高而中后期 (12．48h)低的消化特性与产气法规律并不一致，说明象草中的蛋白质和 碳水化合物在瘤胃内的消化生理是不同的。纤维组中各成分降解率随消化 时间的延长而逐步升高；粗纤维降解率较低，48h降解率为26．78％，其规 律与体外产气规律一致。 3．桂牧一号象草氨基酸降解率的研究，表明象草氨基酸浓度在降解后有上 升趋势，经瘤胃液降解后氨基酸占粗蛋白的比例上升。象草瘤胃液培养12 小时，Arg、Lys、His、Val、Ile和Leu降解率分别为9．2％、4．76％、0．41％、 8．07％、9．2％、9．46％，说明其消化前期能抵抗瘤胃液降解。 综合分析表明，桂牧一号象草在瘤胃中12h内消化有受阻现象，主要 表现为产气量偏低，粗纤维和部分氨基酸(Arg、Lys、His、Val、Ile和Leu) 降解率受阻，这可能就是象草在反刍动物中饲养效果不理想的原因。不同 来源瘤胃液的体外产气试验表明，长期饲喂象草有助于消除受阻现象和提 高反刍动物对象草的消化率。 关键词：桂牧一号象草体夕}消化营养价值消化规律 II 万方数据 STUDY STUDY oN THE DIGESTIoN CHARACTERISTICS oF PENNISETUM PURPUREUMCV．GUIMU．1 IN VITRO AB STRACT Elephant grass has been spread in southern province of China，as its hi曲 yield and nutritional value．Guimu一1 was cultivated by Guangxi Institute of Animal for many years．The quality and forage yield are higher than their parents．Many years of application promotion，find that the result of feeding ruminant are not satisfactory．In order to find out the factors that influence the Guimu一1 and make better use of Guimu一1’S feeding value，this research is to assess Guimu--1 by using gas production method and static fermentation·-nylon bag method in vitro．The present research included three experiments listed as follow： 1．Evaluation of Guimu-1’S gas characteristics of different rumen fluid by gas production method in vitro of within 48h fermented．The sources of r1．1men fluid，the goat feed Guimu-l(goat rtlmen fluid I)，goat the goat feed conventional concentrate and roughage(1 umen fluid II)，the COW feed III 万方数据 conventional conventional concentrate and roughage(COW rumen fluid III)，respectively．The gas production after 3h，6h，1 2h，24h，48h in vitro digestion，the results were showed as follows：Along with the extending cultivation time，the gas pr