缓冲体系对丙酮丁醇梭菌发酵生产丁醇的影响-生物工程专业论文.docxVIP

缓冲体系对丙酮丁醇梭菌发酵生产丁醇的影响Effect 缓冲体系对丙酮丁醇梭菌发酵生产丁醇的影响 Effect of Buffer Solution on Butanol Fermentation by Clostridium acetobutylwum Abstract Recently，global concerns on sustainable supply of crude oil and environmental issues associated with the over-consumption of petroleum-based products，particularly transportation fuels，have revitalized biofuels production．Among these alternatives，bio—butanol as an advanced biofuel has attracted worldwide attention．However,the major problems associated with ABE fermentation include selection of low—cost feedstocks，multiple end-products and by-products，poor stress tolerance，low butanol production，productivity and yield，and high cost of butanol recovery，which seriously hinder the scale—up bio-butanol industrial production．Thus，there is a necessity for developing an efficient bioprocess engineering strategy for addressing these challenges． In the past decades，intensive studies have been carried out on the efficiency of ABE fermentation，which has been demonstrated to inevitably depend on not only environmental pH and acids(butyrate)Ie—assimilation but also the essential availability of nutrients such as metal ions in media．Therefore，in this study，a novel buffer system including MnC03，CaC03 and butyrate Was further developed via Response Surface Optimization．As a consequence， the environmental pH le‘vel could be regulated to some extent by the buffering effect of MnC03 and CaC03，coupled丽th supplementation of micronutrient metal ions and butyrate， which were of great importance to butanol biosynthesis． In this study，several experiments were first performed for single—factor testing，whereas MnC03，CaC03 and butyrate were thus chosen as buffering alternatives in ABE fermentation (in 250mL screw-capped bottles containing 1 00mL fermentation medium)using sugar mixture(glucose／fructose=1：4)as carbon SOurCes simulating the hydrolysate of Jerusalem artichoke tubers via Response Surface Optimization method．The Response Surface Optimization results s