加减驻景方对氪激光诱导的豚鼠脉络膜新生血管干预作用的研究-中西医结合临床专业论文.docxVIP

青海大学 青海大学 2010 级攻读硕士学位研究生毕业论文 PAGE PAGE 4 英文摘要 Study of choroidal neovascularization treated by administration with modified Zhu Jing prescriptions. Abstract Objective: To explore the effectiveness of treatment with traditional medicine in CNV（choroidal neovascularization）and offer a therapeutic schedule of traditional medicine in clinic. Methods: Fifty of 3-week old female guinea pigs with CNV induced by laser photocoagulation were randomly assigned to five groups with ten respectively in each group: Prevention group, higher dosage group, moderate dosage group, lower dosage group and model groups. All lefteyes received repeated multi-wavelength krypton laser photocoagulation treatment (wave-length: 532 nm; laser power: 120-180 mW; spot diameter: 50μm; exposure time: 0.1s). The prevention group was administrated by Chinese medicine gavage before the photocoagulation, while the other groups were administrated after 24 hours of photocoagulation. Fundus photography and indocyanine green angiography （ ICGA ） were performed on the 21th day after photocoagulation. Following that, fluorescein isothiocyanate (FITC ) was injected into guinea pigs by ear vein. Afterwards, the animals were sacrificed immediately and the eyes were enucleated and processed for flat mount of choroidal neovascularization. Results: ICGA showed that the model group and the higher dosage group had higher proportion of CNV occurred in laser spots than the others. The model group had the biggest permeation area. Flat mount of choroidal neovascularization had the same result as the ICGA. Conclusion: Modified Zhu Jing prescriptions has an positive effect to CNV induced in guinea pigs. Chinese medicine prevention groups showed the best efficacy than other groups. The moderate dosage is the most suitable dosage to inhibit the CNV, while the higher dosage is the worst one. Keyword: Modified Zhu Jing prescriptions; choroidal neovascularization; multi-wavelength krypton laser 引 言 （立题依据） 课题来源