吉祥草质量标准及指纹图谱分析-农药学专业论文.docxVIP

PAGE PAGE 2 因此,将凯提皂苷元作为指标成分，对吉祥草的质量控制具有重要意义。凯提皂 苷元质量在5~30 μg之间线性关系良好，γ= 0.9993，平均回收率为98.04%，RSD =2.6% (n=9)同时，对不同产地的吉祥草中的指标性成分进行含量测定，为建立 吉祥草药材科学合理的质量控制方法和实现其规范化种植提供理论依据。 第四部分：吉祥草指纹图谱的研究 本部分首次对吉祥草药材采用HPLC-ELSD建立化学指纹图谱方法，并对13 批来自全国各地的样品进行了指纹图谱分析，其中贵州地区样品之间的相似度大 于0.9，其余各地样品与贵州地区样品的差异主要表现在指纹图谱的各峰的峰形 及相对高度上，导致相似度较低，说明了吉祥草化学成分受地区差异影响明显， 通过对吉祥草高效液相指纹图谱的分析找出吉祥草特有的指纹特征，以此来对吉 祥草真伪优劣进行鉴别和评价。 第五部分：吉祥草药材中重金属元素的含量测定与一般质量检查 本部分采用原子荧光光度法和原子吸收分光光度法测定了吉祥草药材中的 Pb、Cd、Hg、As、Cu五种重金属元素，其回收率为95.81%~100.52%之间，RSD 3%。结果表明，吉祥草中含有Pb、Cd、Hg、As、Cu等重金属元素，其中，贵州 地区吉祥草中Cu含量均大于20 ppm，临床使用必须高度重视。中药的一般质量 检查也非常重要，因为中药的一般质量直接关系到中药的疗效，中药的储存过程 对中药的一般质量要求相当严格。因此，中药的一般质量研究在中药的质量控制 过程中是必不可少的。对本部分的一般质量研究包括 :水分测定、浸出物测定、 总灰分测定、酸不溶性灰分的测定。 关键词: 吉祥草；质量标准；指纹图谱；高效液相色谱法 Abstract Reineckea carnea (Andr.) Kunth., belonging to Liliaceae family, called Jixiangcao in Chinese has been used as one of the traditional Chinese medicines (TCMs) and is officially listed in quality standards for Chinese herbal medicine and national medicines of Guizhou Province. A series of researches about Jixiangcao have been taken and some useful conclusion had been made. However, there are few researches about the quality control of Jixiangcao, so it is very necessary to study much about it. This subject mainly studies on the standard of the quality control of Jixiangcao by means of modern analysis technology, and there are five Parts in this program: Part one: Establish a method by using thin layer chromatography (TLC) It is the first time to establish a method by using thin layer chromatography (TLC) to identify Jixiangcao, including 10 batches of Jixiangcao which come from different places. Part two: Determine the content of the general saponins of Jixiangcao Pharmacology studies proved the general saponins of Jixiangcao has been used as relieving a cough, abolishing phlegm, anti-inflammatory. This part mainly determined the content of the general saponins of Jixiangcao by UV. The calibration curve was linear over the range o