黄姜皂素清洁生产工艺的研究-生物化学与分子生物学专业论文.docxVIP

实验表明：清洁生产工艺总甙体积减少为原来物料的lO％以下。酸用量减少了 实验表明：清洁生产工艺总甙体积减少为原来物料的lO％以下。酸用量减少了 90％、废水排放量减少了75％、废水的COD、色度大大降低，而且将皂素收率提 高了6．46％；还提高了产品纯度，产品洁白，熔点198℃，达到中华药典的出厂标 准。 乙醇取代汽油提取皂素新工艺最佳工艺条件如下：(1)109干水解物皂素索氏 抽提时间为6h，(2)l撑试剂的最佳用量为200ml，(3)硫酸钙的用量以39为最 佳，(4)活性炭按109水解物加入0．49。乙醇取代汽油提取皂素的新工艺，所得皂 素收率、品质、成本方面均优于汽油法提取。用可再生资源取代了不可再生资源， 还提高了生产的安全性。 关键词：黄姜； 皂素； 总甙； 水解； 提取； 清洁生产工艺 ⑨ ⑨ 硕士学位论文 MASTER‘S THESIS Abstract The wastewater from diosgenin production of Dioscorea Zingiberensis C．H．Wright was of great acidity，high organic matter concentration and carbohydrates produced in the process of asylums hydrolyzed．The rivers which the wastewater poured into directly without any treatment from the diosgenin factories were polluted seriously,which had been a hard nut to crack of the healthy development of the industry．In this paper,the new technology of extracting diosgenin was studied here in detail．the cleaner production technology Was used firstly tO extract Dioscorea Zingiberensis CH．Wright and petrol was replaced by ethanol to extract diosgenin． The five different cleaner production technologies were investigated：extracting diosein directly,extracting dioscin a氩elnatural fermentation,extracting dioscin after solid-state fermentation,extracting dioscin after two-enzyme hydrolysis，extracting dioscin after fermentation by Aspergillus niger．Results were shown that the extracting diosein after natural fermentation Was the best cleaner production process． The main results of cleaner production process were as follows： I．40 grams of dry Dioscorea Zingiberensis C．H．Wright powder(about 1 00 grams of flesh Dioscorea Zingiberensis c。H。Wright)were naturally fermented for 24 hours． 2．The optimal extracting dioscin time was 6 hours． 3．1 0 grams of dioscin were hydrolyzed by 20 ml of I mol／L sulfuric-acid． 4．The optimal acid hydrolysis time Was one hour． 5．The best Diosgenin extraction time Was 4 hours． 6．3 grams of activated carbon was added into 1 0 grams total dioscin． The results showed：the volume of tota