利用A-PAGE研究谷子籽粒蛋白质多态性-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2009, 35(7): 1374−1378 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.01374 利用A研究谷子籽粒蛋白质多态性 * 杨延兵 张华文 秦 岭 王海莲 管延安 段乃彬 张 晗 , 250100 : A 24 , , ; 20 , 4 , A , 7~10 , 5 , 2~5 , A, , , , , : ; ; ; A Polymorphism Analysis of Seed Storage Proteins in Foxtail Millet (Setaria italica L. Beauv.) with A * YANG Yan-Bing, ZHANG Hua-Wen, QIN Ling, WANG Hai-Lian, GUAN Yan-An , DUAN Nai-Bin, and ZHANG Han Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China Abstract: The polymorphism of water-soluble protein, salt-soluble protein, acid-soluble protein and prolamin was analyzed by Ain 24 foxtail millet varieties from different ecological zones. The results revealed that the banding patterns of the wa- ter-soluble protein in different millet varieties were similar to those of the acid-soluble protein. There existed over twenty bands of the salt-soluble protein in each millet variety, and only four varieties contained polymorphic bands, showing that bands of the water-soluble protein, the salt-soluble protein and the acid-soluble protein obtained by Aare not available to use in millet varieties identification. There existed a certain degree of heterogeneity in millet prolamin bands. Sev