活血利水之散血明目片对视网膜静脉阻塞后视网膜保护作用的实验分析-中西医结合临床(眼科)专业论文.docxVIP

中文摘要目的：探讨活血利水之散血明目片对视网膜静脉阻塞(retinal 中文摘要 目的：探讨活血利水之散血明目片对视网膜静脉阻塞(retinal vein． occlusion，RVO)的治疗作用及防治并发新生血管的作用机理，为ll占床应 用提供理论依据。 方法：40只El本大耳白兔随机分为4组，每组10只兔20只眼， 分为A组健康空白纽、B纽模型组、C组血塞通治疗组、D纽散血明目片 治疗组。采用光化学法建立RV0模型，连续灌胃，实验后第7d，21d分 别处死家兔，检测视网膜中超氧化物歧化酶(SOD)、丙二醛(MDA)、 血管内皮生长因子(VEGF)浓度，光镜下观察视网膜各层结构变化。 结果B、c、D三组60只眼成功建立了RVO模型。遣模后第7d， 各造模组眼底均见出血，D组与A、B、C三纽比较：SOD活力明显高 于其B、C两组，低于A组，且差异有显著性(P0．01)；MDA、VEGF 含量明显低于B、c两组，高于A组，有显著性差异(P0．01)；遣模后 第21d，D组眼底出血已完全吸收，B、C两纽仍可见到出血。各检测 指标结果同7d。光镜下观察7d、21d时D组视网膜各层结构病理变化 均轻于B、C组。 结论活血利水之散血明目片能促进RVO后出血的吸收，改善视 网膜组织缺血缺氧的状态，提高其抗氧化能力，抑制VEGF在视网膜面 的高表达，进而治疗RVO，防治RVO后并发新生血管，保护视网膜。 关键词视网膜静脉阻塞(RVO)；活血利水法；散血明目片；兔； 超氧化物歧化酶(SOD)；丙二醛(MDA)；血管内皮生长因子(VEGF) Experimental Experimental Study of SanXue MingMu TABLET on Protecting Retina after Retinal Vein Occlusion Postgraduate：Han Oi Tutor：Peng qinghua Hunan College of Traditional Chinese Medicine (Changsha China，410007) Objective：To investigate the effects of Sanxue Mingmu Tablets(SMT) on treat retinal vein occlusion(RVO)and the action mechanism of controls the regeneracy blood vessel．Provides the theory basis for the clinical practice． Method：40 rabbits were randomly into 4 groups，with 10 in each． Ten animals in group A(GA)were taken as healthy controls，and the other rabbits in group B，C and D were fed with normal saline，positive controlling dmg and SMT respectively,then prepared as RVO model by photochemistry．After the experiment 7th and 21st day to kill the rabbits respectively．To detect the density of SOD、MDA and VEGF in the retina，and the morphological variations were observed under light microscope． ResuIts：The RVO model have established SuCCesses in 60 eyes．We can see haemorrhage at eyeground of every group after the model have established successes 7d．Compare D group with A，B，and C groups：The vitality vitality of SOD is obviously higher than B、C groups，the content of MDA is obviously lower than B、C groups，the content of VEGF is obviously lower than B，C group，Also the difference was significative(P0．0