鸡源乳酸杆菌的分离鉴定及保存技术研究-动物营养与饲料科学专业论文.docxVIP

Study Study On Isolation，Identification and Conservation of Lactobacilli From Poultry Auor．Zhang Dongyan Advisen：Zhao Guoxian(Professor) Ji Haifeng (R∞∞rchef) Major．Animal Nutrition and Feed Science Abstract In order to prepare the best probiotic of poultry,the experiment isolated five Enterococcua and two Lactobacilfi from the blind intestinal mocous and feces of healthy Beijing You Fowl．According to the experiments of OD600咖and pH，we selected 1 s仃ain as the better lactobacilli．and confirm the best culture condition．Then， the experiment carried out some experiments including optimizing culture medium， cultivating in 50 litre fermenter,freeze-dyring with different cry-protective agent，and SO on．At last,we made the production of the freeze-dry powder of M8 in broilers’feed in order to investigate its effect on the microflora ofbroilers．The results showed： (1)4l strains bacterias species from the blind intestinal mOcolL‘J and feces of heMthy Beijiing You Fowl．T110ugh the observation of colonial morphology and Gram staining,7 strains were identified physiologically and biochemistrically according to the diagnostic characteristics ofbacteria and were preliminarily defined as Lactic acid bacteri扎The result showed：M1、M2 were defined as E．SCecorum；F5、F8、FI l were defin。d as E．faecalis；M3 Was defined as L brevis and M8 Was defined as L． pentosus． (2)The experiment got the growth COIVes and pH of Lactobacilli M3 and M8 in breezy and sealed bottles．OD甜om of M3 reached to 1．154 and pH reached to 4．16 from 5．86 in breezy bottles，and in the sealed bottles，OD鲫姗reachcd to 1．157 and pH reached to 4．14 from 5．85．On the other hands．OD600Ⅲof M8 reached to 1．Ol l and pH reached to 4．36 from 5．64 in breezy boales，and in the sealed bottles，ODeom reached to 2．083 and pH reached to 3．42 from 5．64．Therefore,M8 Was the better Lactobacilli and its optimum culture condition Was in sealed bottles． (3)We also got the growth OD6∞‰and pH of M8 in sealed bottles with different levels of inulin