环磷酰胺、秋水仙碱与丰富环境联合应用对急性脑梗死大鼠感觉运动功能影响的研究-神经病学专业论文.docxVIP

四”i大学临床医学颈士专业学位论文环磷酰胺、秋水仙碱与丰富环境联合应用对急性 四”i大学临床医学颈士专业学位论文 环磷酰胺、秋水仙碱与丰富环境联合应用对急性 脑梗死大鼠感觉运动功能影响的研究 研究生张振铎 导 师罗祖明教授 摘 要 圈的：探讨环磷酰胺、秋水仙碱与丰富环境联合应用对急性脑梗戮 大鼠感觉运动功能和微管相关礞白(Microtuble associated protein．2， MAP-2)、黢矮终缀羧缝蛋自(glial fibrillary acidic protein，GFAP)羯戆 表达的影响。 方法：采用改良的Zea-Longa线枪法制铸大鼠大脑中幼脉缺庇再灌 注模羹(MCAO Ire．)。成年健康瓣性SD大鼠40只随机分为4组，每组 lO只：A缀给予臻磷酸黢+敬承铀碱，嚣予事富环境中；器缀给予琢磷 酰胺+秋水仙碱，壁～般环境中；c组不绘环磷醮胺+秋水蚀碱，爨丰富 环境中；D组不给环磷酰胶+秋水仙碱，置一般环境中。给药组在缺血再 灌注后111,12h，24h给予环磷酰胺+秋水仙碱。各组大鼠在缺血再灌注后 24h，l两，2攒，3蠲，4璃透行棒经荨亍为学溅试谱分。廒霜免疫组化的方法 观察各缀MAP-2，GFAP敬阳性袋达。 结果：神经行为学测试评分B组、C组之间在MCAOI／R后4周无 四川大学临床医学硕士专业学位论文显著性差异(PO．05)但均较D组有显著性差异(P0．05)。A组较B组、 四川大学临床医学硕士专业学位论文 显著性差异(PO．05)但均较D组有显著性差异(P0．05)。A组较B组、 C组有显著性差异(P0．05)。MAP．2的阳性表达B组、C组间无显著性 差异(P0．05)但较D组均有显著性差异0O．05)；A组较B组、C组有 显著性差异(pO．05)。GFAP阳性表达各组间无显著性差异(PO．05)。 结论：环磷酰胺、秋水仙碱和丰富环境均可提高急性脑梗死大鼠感 觉运动功能。二者联合干预较单一干预措施更为有效。 关键词：脑梗死；环境因素；可塑性；环磷酰胺；秋水仙碱 巴业查堂堕壅垦堂堡圭童些堂堡垒苎The 巴业查堂堕壅垦堂堡圭童些堂堡垒苎 The experimental study of cyclophospamid，coichicine combined with enriched environment influences the sensorimotor function of acute middle cerebral artery occlusion in rats Postgraduate：Zhang zhenduo Tutor：Luo zuming Abstract objecfive To investigate the sensorimotor functional influenced by cyclophosophamid，colchicine combined with enriched environment aRer acute cerebral infarction in rat and the positive expression of microtuble- associated protein-2(MAP一2)and glial fibrillary acidic protein(GFAP) Methods The models of middle cerebral artery occlusion ischemia／ reperfusion(MCAO I瓜、were established with the Zea-longa suture method． 40 adult males SD rats were divided into 4 groups randomly：group A，the rats were given cyclophosphamid and colchicine and kept in an enriched environment；group B，rats were given cyclophosphamide and colchicine but kept in a common environment；group C，rats not given cyclophosphamide or colcllicine but kept in ail enriched environment；group D，rats not given cyclophosphamide or colchjcine and kept in a common environment． Cyclophosphamid and eolcllieine w