脊尾白虾繁殖生物学分析-海洋生物学专业论文.docxVIP

摘要 摘要 脊尾白虾繁殖生物学研究 脊尾白虾繁殖生物学研究 III III 因表达量的变化趋势，与其对应的卵巢发育基本一致。这也从另一方面证明了 卵黄蛋白原基因对卵巢发育的调控作用。 关键词：脊尾白虾；中国对虾；法尼酸甲基转移酶；卵黄蛋白原；繁殖 Abstract Exopalaemon carinicauda has sone characterics such as long breeding season,fast growth and strong adaptability to environment, etc. In recent years, with the development of the mariculture area to low salinity, morphological and white shrimp farming production was increased rapidly, often mixed with other Marine economic species breeding. For the study of morphological and white shrimp in the regulation mechanism in the process of reproduction, the paper first cloned FAMeT genes, and were measured in three consecutive ovarian development FAMeT gene expression in the morphological and white shrimp. To end white shrimp penaeus chinensis and ridge at the same time in the three consecutive breeding vitellogenin gene with the ovaries tissue section make a comprehensive analysis, this paper expounds the tail white shrimp penaeus chinensis and ridge breeding. The specific research results are as follows: The morphological and white shrimp FAMeT gene cloning In order to study method, acid methyl transferase (FAMeT) on morphological and white shrimp role in the development of ovary, using reverse transcription PCR and cDNA end rapid amplification technology (RACE) cloning get the length of the tail ridge white shrimp FAMeT cDNA sequence (GenBank login number: AHB23861), it includes a 76 bp of 5 non coding region (untranslated regions, UTR), a 712 - bp 3 noncoding region and a 831 - bp open reading frame (open reading frame, ORF). Encoding 276 amino acids, 3 UTR with typical plus tail signal AATAAA and Poly real (A) tail. Sequence analysis found that the sequence of amino acid sequence has the characteristics of FAMeT CF (CPAMD8 / FAMeT) area, thus can judge the cDNA sequence is ridge tail FAMeT gene sequences of white shrimp. Use of real-time fluorescent quantitative PCR (qRT - PCR) method is analyzed in different tissues, different amount of e