玫瑰挥发油抗念珠菌活性的体外研究-皮肤病与性病学专业毕业论文.docxVIP

兰州大学硕士学位论文克柔念珠菌2株(3．28％)及季也蒙念珠菌1株(1．64％)。 兰州大学硕士学位论文 克柔念珠菌2株(3．28％)及季也蒙念珠菌1株(1．64％)。 2．纸片扩散法药敏试验中玫瑰挥发油(30％、20％、10％和5％)的抑菌圈直 径为28mm、24mm、18mm和14mm；其3种单体化合物(15％香叶醇、15％t3．香 茅醇和15％丁香酚)的抑菌圈直径分别为22mm、27mm和18mm；空白对照 I％DMSO为0mm；阳性对照0．2％FCZ的抑菌圈直径为20ram。 3．玫瑰挥发油对临床分离念珠菌均有较强的抗菌活性，且各菌种问差异无显著 性；两种方法测定玫瑰挥发油对念珠菌的MIC值一致率为93％，二者无显著性差 异(P=O．18)。 4．玫瑰挥发油与氟康唑联用对氟康唑敏感株及耐药株均表现出较好的协同和 相加作用，无拮抗作用；两组间分数抑菌浓度指数(FICI)差异无显著性(尸=0．545)。 5．三种单体化合物对临床分离念珠菌均有较强的抗菌活性，平均MIC、MFC 分别为：香叶醇424．70／tg／mL与505．3弘g／mL，D一香茅醇411．7陬g／rnL与 455．78／zg／mL，丁香酚579．1舡g／mL与842．6似g／mL。 结论 1．玫瑰挥发油及其3种单体化合物对临床分离念珠菌均有不同程度的抗菌作 用。 2．常量稀释法与微量稀释法有良好的一致性，且微量法更具有操作简便、节约 药物和试剂、减少污染机会、重复性好等优点，更适于临床实验室应用。 3．玫瑰挥发油与氟康唑联用对念珠菌具有体外协同抑菌作用。 4．香叶醇、B．香茅醇和丁香酚为玫瑰挥发油抗念珠菌的有效单体成分。 5．玫瑰挥发油及其3种单体化合物抗念珠菌作用方式可能为杀菌。 关键词 玫瑰挥发油；念珠菌；香叶醇：13．香茅醇；丁香酚；氟康唑；抗微生物敏感性 试验 II 兰州大学硕士学位论文Study 兰州大学硕士学位论文 Study on Anti—candidal Activity of the Essential Oil from Rosa rugosa in vitro ABSTRACT objective To determine the anti-candidal activities of the essential oil from Rosa rugosa (EORR)and its three components(geraniol，B-citronellol and eugen01)，and to investigate the combined effect of RORR with fhrconazole against Candida spp．in vitro， SO as to set up the foundation for studying its antifungal mechanism and clinical combination therapy in the future；meanwbS!e，co_mpare the results of two antifungal susceptibility tests of NCCLS-M27一A． Methods 1．Sixty-one Candida strains were isolated from varieties of clinical specimens and then identified by germ tube test，chlamydospore test，and API 20C Aux system， respectively． 2．The anti-candidal activities of the essential oil from Rosa rugosa and its three components were proved by disc diffusion method． 3．The minimum inhibitory concentration(MIC)and the minimum fungicidal concentration(MFC)of the essential oil from Rosa rugosa to 61 clinically isolated Candida strains were tested by NCCLS—M27-A microdihrtion and macrodilution method， respectively．Meanwhile fiuconazole and amphotericin B were used as quality control drugs．