棉铃虫核多角体病毒的传播动态及含sod重组病毒的构建-微生物学专业毕业论文.docxVIP

Transmission Transmission dynamics ofHelicoverpa armigera Nucleopolyhedrovirus and construction of a recombinant virus expressing superoxide dismutase ZHOU Ming-zhe(Major in Microbiology) Directed by Prof．SUN Xiu—lian ABSTRACT There are two chapters in this thesis． In chapter one we reported the horizontal and vertical transmission dynamics of wild-type Heficoverpa arm枷ra single·nucleocapsid nucleopolyhedrovims(HaSNPV) and a genetically modified variant限aSNPV-AaIT)with enhanced speed of action through the expression of all insect—selective scorpion toxin“art)．In caged field plots，horizontal transmission ofboth HaSNPV v耐ants was greatest when inoculated 3“instar larvae were used as infectors．intermediate with 2“o instar infectors and the lowest with 1“instar infectors．Transmission Was greater at a higher density of infectors(1 per plant)than at a lower density(1 per 4 plants)；however,the transmission coefficient(proportion of new infections per infector)was lower at the higher density of infectors than at the lower density．The use of HaSNPV-AalT exhibited in a significantly lower rate oftransmission than HaSNPV-wt，both in caged field plots and in the open field．In the laboratory,the vertical transmission of HaSNPV：AaIT from infected females to offspring WaS 16．7 4-2．1％．which Was significantly lower than that of HaSNPV-wt(30．9％士2．9)．No vertical transmission from males could bc demonstrated．Likewise．in the field,vertieal transmission of HaSNPV-AaIT(8．4士1．1％)was significantly lower than of HaSNPV-wt(12．6士 2．O％、．The results indicate that the recombinant virus will spread less in H armigera population than the wild-type virus．As a result，the recombinant virus is impaired in its ability to maintain itself over multiple insect generations．potentially affecting H negatively negatively its long term effectiveness compared to the wild—type virus，but contributing positively to biosafety． Some kind of phenolase secreted by co,on glands，which generate free radicals， dramaticall