芒果三芪肺纤方对博莱霉素致小鼠肺纤维化治疗作用及其机制研究-中药学专业毕业论文.docxVIP

0.01-0.05）；HE和Masson染色观察显示芒果三芪肺纤方组可以减轻肺泡炎 损伤和抑制肺纤维化病变程度（P＜0.01）；而且能显著性的降低HYP的表 达水平（P0.01）。芒果三芪肺纤方组都能够调节肺纤维化小鼠体内的自 由基代谢，显著提高模型小鼠体内总SOD活力（P0.01），并显著性的降 低MDA含量的表达（P0.01），ELISA法检测IL-β的含量表达显著性的降 低(P0.01)。 （2）芒果三芪肺纤方组、益气化痰方组、益气活血方组、活血化痰 方组小鼠体重较模型组均显著性增加（P＜0.01-0.05），芒果三芪肺纤方 组和益气化痰方组效果最好；HE和Masson染色观察显示各给药组可以减 轻肺泡炎损伤和抑制肺纤维化病变程度（P＜0.01），；而且能显著性的降 低HYP的表达（P0.01）。各给药组都能够调节肺纤维化小鼠体内的自由 基代谢，显著性地提高小鼠体内总SOD活力（P0.01），并显著性的降低 MDA含量的表达（P0.01），ELISA法检测表明各给药组IL-β的含量表达 显著性的降低 (P0.01)。 （3）HE和Masson染色观察显示芒果三芪肺纤方组可以明显的减轻 肺泡炎损伤和肺纤维化程度（P＜0.01）；免疫组化的结果显示芒果三芪肺 纤方组显著降低TNF-ɑ的表达 (P0.01)；RT-PCR的结果显示芒果三芪肺 纤方组显著降低TNF-ɑ mRNA的表达 (P0.01)。 结 论：芒果三芪肺纤方组都可以通过以下三个方面发挥作用：（1） 提高总SOD活力，降低MDA水平而发挥抗自由基损伤作用；（2）通过降 低HYP的含量影响胶原的产生而减少纤维蛋白的形成；（3）通过调节IL- β的表达和TNF-ɑ含量表达对炎症因子释放的抑制而发挥抗炎作用。 关键词：芒果三芪肺纤方；芒果苷；黄芪多糖；三七总皂苷；肺纤维化； Study on preventive effect and mechanisms of Feixian II prescription to inhibit bleomycin-induced pulmonary fibrosis in mice ABSTRACT Objective: To study the preventive effect of Feixian II prescription to inhibit bleomycin-induced pulmonary fibrosis in mice.Seeking effective methods of preventing drug-induced pulmonary fibrosis to alleviate the suffering of patients. Methods:(1)The study of therapeutic effect of bleomycin-induced pulmonary fibrosis in mice with Feixian II prescription:Mice were prepared with intranasal instillation infusion a dose of 6mg/kg bleomycin.Healthy mice were randomly divided into five groups: A control group, B model group, C the positive drug group,D Feixian II group.Each administered group start giving appropriate intervention after 14d of modeling,every mice in each group were sacrificed after 14 days.the lung tissues were collected. Colorimetric method was used to measure the content of SOD, MDA, hydroxyproline (HYP) in lung tissue and SOD, MDA in serum. The left lung was used to make pathological sections which were stained with Haematoxylin-Eosin and Masson dyeing for pathological diagnosis. ELISA method was used to assay the level of IL- 1βin lung. （2）The study