罗非鱼鱼皮胶原蛋白的提取及其功能特性的研究-食品工程专业毕业论文.docxVIP

罗非鱼鱼皮胶原蛋白的提取其及功能特性的研究为0．03％、pH3．O-3．5的ASC起泡性高达128％，泡沫稳定性良好；乳化性为 罗非鱼鱼皮胶原蛋白的提取其及功能特性的研究 为0．03％、pH3．O-3．5的ASC起泡性高达128％，泡沫稳定性良好；乳化性为 93％，放置4h后仍能保持50％以上的乳化性，可见其乳化稳定性良好。这些功 能特性有利于ASC作为基料或乳化剂、保湿剂应用于保健食品加工及化妆 品生产中。 关键词：酸溶胶原蛋白、酶促酸溶胶原蛋白、氨基酸组成、理化性质、功能特性 罗非鱼鱼皮胶原蛋白的提取其及功能特性的研究Study 罗非鱼鱼皮胶原蛋白的提取其及功能特性的研究 Study On Isolation and Characterization ofCollagens Abstract The output of Oreochromis niloticus occupies first place in freshwater fish． During processing sliced tilapia，great quantities ofthe byproduct such as skin，bones and fins are produced．Among these wastes，most is skill Therefore，this thesis studies On isolation collagens from the skin oftilapia and their characterization are analyzed． The results indicate that the crude protein and collagen content ofthe skin offilapia which is obtained from fish-processing factory arc up to 33．14％and 27．8％．respectively,on the basis ofwet weight．The content ofcollagen account about 83．9％of the crude protein．After removing noncollagenous proteins with O．1mol／L NaOH and fat诵m lo％(v／v)butyl alcoh01．The yields ofASC and PSC ale 39．4％ and 44．6％．respectively,on the basis of dry weight．This shows that collagen in the skin could not solve completely in acid．At the sanle time,the yield ofPSC WflS slight higher than that of ASC．Therefore．most collagen in the skin is moncollagen．弧e av(tin(3 acid comnogiticm of ASC and PSC are similar．ASC and PSC are rich in glycine(35．6％and 33．8％)，The amounts of imino acids，proline and hydroxyproline inASCandPSCare210和217 residuesperl000 residues．respectively．The distinct absorption are obtained at 220 um and 222nm；respectively．This result is in accordance with the characterization of the type I．ASC and PSC show transition curves with maximum temperatures(％茹of 3 1．9TC in O．05M acetic acid，which is lower 1 0℃than that of calf skin collagen．Maximum solubility in O．5M acetic acid is observed(O．75mg／mL and O．78mg／mL)at pH 3 and pH 4 fur ASC and PSC， respectively．Solubility reaches the minimum when the pH is 7．A sharp de