慢性心肌缺血动物模型的建立及血管新生的基础研究-内科学专业毕业论文.docxVIP

复}]大学 复}]大学 硕士论文 慢性心肌缺血动物模型的建立及血管新生的基础研究 摘 要 研究背景：血管新生是缺血性心脏病治疗的新途径。本研究旨在探索建立猪的 慢性心肌缺血动物模型，并通过选择性冠状动脉造影及病理检查来证实。 方法：选用小型猪，复合麻醉、开胸、分离左回旋支近段，并安置Ameroid环， 利用环内物质吸水均匀膨胀，造成动脉慢性闭塞。第3周及第5周行冠状动脉 造影，观察血管狭窄及侧支血管生成的程度。5周后处死动物，行苏木精·伊 红染色及免疫组化检查，观察组织病理变化及心肌新生血管密度。 f结果：最终连续造模成功4例。冠状动脉造影显示，第3周左回旋支近段狭窄 岫显，第5周完全闭塞并生成侧支血管II级。心肌组织的病理改变为慢性心肌 ＼ 缺血所致，缺血区新生血管密度较梗死区及正常组织区明显增加。P j 结论：成功建立慢性心肌缺血动物模型，实验结果均一、稳定，手术成功率高。 模型已生成一定程度的侧支循环，可用于血管新生的基础研究。 关键词：慢性心肌缺血动物模型血管新生侧支循环 复旦盔堂 复旦盔堂 堡主笙蔓 Establishment of Animal Model with Chronic Myocardial Ischemia and Preliminary Research on Angiogenesis Abstracts Backgrounds：Angiogenesis is a new strategy of treatment for ischemic heart diseases．The aim of research is to develop all animal model of chronic myocardial ischemia in minipigs and testify via selective coronary arteriography and pathological study． Methods：Minipigs were used in the study．Following anesthesia，thoracotomy, dissection of left proximal circumflex coronary artery,and placement of Ameroid constrictor,chronic coronary artery occlusion was set up by the material which in constrictor sucked up moisture and expanded well-distributedly．Coronary arteriography was performed to evaluate stenosis of ipsilateral vessels and establishment ofcollateral angiogenesis at week 3 and week 5．At week 5，the animals were sacrificed，Hematoxylin-Eosin staining and immunohistochemistry were performed to evaluate pathological changes and the density of angiogenesis in myocardium． Results：Four animals have been induced to chronic myocardial ischemia．Obvious stenosis of left proximal circumflex coronary artery was observed at week 3，and total occlusion with grade II collateral angiogenesis was found at week 5．The pathological changes of myocardium were due to chroni