慢病毒介导的RNAi沉默RhoA基因对人卵巢癌细胞侵袭转移影响的实验分析-妇产科学专业毕业论文.docxVIP

广州医学院硕士论文 PAGE PAGE 10 示，干扰组细胞中 RhoA mRNA 和蛋白的相对表达率分别为（30.70±5.40 ）%、 （32.08±5.46）%，通用阴性对照组为（95.90±6.53）%、（60.58±6.21）% ， H08910 组为（100.0±4.37）% 、（59.25±4.52）% ，干扰组与其他两组对比 差异有统计学意义（P＜0.05），表明成功构建了 RhoA 基因稳定沉默卵巢癌细胞 株。 4、人 RhoA 基因稳定沉默卵巢癌细胞株的体外增殖能力、侵袭和迁移能力、诱导 血管形成能力、黏附能力较对照组明显减弱，差异有统计学意义（P＜0.05）。 结论 1、成功构建针对 RhoA 基因的慢病毒干扰载体，可长久稳定地抑制卵巢癌细胞 HO8910 中 RhoA 基因的表达。 2、沉默 RhoA 基因能抑制卵巢癌细胞的生长及侵袭和转移。 关键词 慢病毒； RNA 干扰；RhoA 基因；卵巢癌；侵袭和转移 Effect of lentivirus- mediated silence of gene RhoA on the invasion and metastasis of the ovarian cancer cell Post-graduated: WenJuan Yang Supervisor：Prof. JiaLi Kang Department of Obstetrics and Gynecology, Guangzhou First Municipal Peoples Hospital Affiliated to Guangzhou Medical College, Guangzhou, 510180,China Abstract Background Relapse and metastasis always result in poor clinic outcome ,many people are died of the relapse and metastasis of the malignant tumors. Ovarian cancer is one of the most severe gynecological malignancies known and has now become the most lethal of all gynecological malignancies in Chinese women.The precise cause of epithelial ovarian cancer estimated to account for about 90% of all ovarian carcinomas is still not known. Most women present late stage of disease at diagnosis and this has contributed to the high mortality rate of between 80 and 90% compared to early stage disease of between 10 and 30%. The overall 5-year survival rate falls below 40% . The etiology and early events in the progression of ovarian cancers are poorly understood. The genetic basis is very complex ,ampliation and/or altered expression of oncogene sand tumor suppressor genes can play an important role in the development of ovarian cancers .A better understanding of the metastasis of the ovary cancer is needed to determine effective therapeutic interventions to either eradicate or slow metastatic outgrowth. Many factors are involved in metastasis through diverse mechanism. Among these oncogene ,RhoA (Ras homologue member A)are found in