基于猪口腔黏膜模型的口含烟烟碱渗透速率的影响因素-烟草科技.PDFVIP

2017 年6 月 烟草科技 Jun. 2017 第50 卷第6 期 Tobacco Science Technology Vol. 50 No. 6 基于猪口腔黏膜模型的口含烟烟碱渗透速率的 影响因素 * 艾明欢，陈超英，郑赛晶，张怡春 上海新型烟草制品研究院，上海市虹口区大连路789 号 200082 摘要：为考察口含型无烟气烟草制品烟碱在口腔黏膜中的渗透行为，设计了透皮扩散池与猪口腔黏膜配 套装置，结合高效液相色谱（HPLC ）法，研究了口含烟所受压力、口含烟pH 及含水率对其所含烟碱渗透 2 速率的影响。结果表明：①在0.70~44.65 μg/mL 质量浓度范围内烟碱标准曲线的线性良好（R = 1.000 ）， 回收率为96.45%~98.96% ；②口含烟中烟碱透过猪口腔黏膜为直线规律，属于膜-贮库型；③在0.5~ 12.0 h -2 2 内，施加6.65 × 10 N/cm 的压力可显著提高烟碱的渗透速率；④含水率处于30%~50%之间时，pH=7.4~ 8.4 的口含烟的烟碱渗透速率明显高于pH=5.3~5.4 的口含烟。因此，可主要通过改变压力和口含烟pH 来调控口含烟烟碱在口腔黏膜中的渗透行为。 关键词：口含烟；口腔黏膜；烟碱；压力；pH ；渗透速率 中图分类号：TS411.1 文献标志码：A 文章编号：1002-0861 （2017 ）06-0033-07 Factors influencing nicotine permeation rate from snus usage based on pig ’s oral mucosa model * AI Minghuan, CHEN Chaoying , ZHENG Saijing, ZHANG Yichun Shanghai New Tobacco Product Research Institute, Shanghai 200082, China Abstract: In order to investigate the nicotine permeation behavior in oral mucosa from snus, a device was designed which incorporated a matching skin permeation diffusion cell and a pig ’s oral mucosa. The effects of pressure, pH and moisture content on the nicotine permeation rate from snus were studied by combining the designed device with high performance liquid chromatography. The results showed that: 1) R2 of the standard curve of nicotine concentration was 1.000 in the range of 0.70-44.65 μg/mL and the recovery ranged from 96.45% to 98.96%. 2) Nicotine permeation from snus through the pig ’s oral mucosa as time presented a linear relations