氯喹抑制放射诱导的自噬对食管癌细胞放射敏感性影响的研究肿瘤学专业毕业论文.docxVIP

PAGE PAGE 10 用 CQ 组（Post-CQ）D0 值为 1.3872 Gy，Dq 值为 0.2040 Gy，N 值为 1.4030，SF2 值为 0.3152，放射增敏比 SERD0 值为 1.4392，α 值为 0.4061， β 值为 0.0638，α/β 值为 6.3652。 结论：放射线可诱导食管癌 TE-1 细胞自噬活性增加及早期凋亡 率增加。放射后使用 CQ 可明显增加食管癌 TE-1 细胞的放射敏感性， 其机制可能与抑制放射线诱导的自噬并增加早期凋亡相关。 关键词：氯喹；自噬；食管癌；放射敏感性。 Influence of Blocking Irradiation-induced Autophagy by Chloroquine on Radiosensitivity of Esophageal Cancer Cells Abstract Objective: Our study is to examine whether blocking irradiation-induced autophagy by chloroquine (CQ) can sensitize esophageal cancer cells TE-1 to ionizing radiation (IR) and its further mechanism. Materials/Methods: Effect of CQ on cell viability of TE-1 cells was determined by MTT methodology. IC10 and IC50 of CQ were calculated by software and the concentration of IC10 was chosen for the following experiments. We designed two groups of different dose of IR alone, different dosing time of CQ combined with IR. After treated with IR, expression of autophagy ralated protein LC3 and Beclin-1 was detected by Western-blot in different time. The formation of acidic vesicular organelles (AVOs) was determined by fluorescence staining with double staining of Lyso-Tracker Red DND-99 and Hochest 33258. Early stage apoptosis of 6h, 24h after different treatment was measured by Annexin-V/PI staining through flow cytometry. Clonogenic survival of TE-1 cells on the treatment with addition of CQ before or after exposure to IR was examined by clonogenic forming assay, and the radiobiology parameters were estimated from the curve fitted by the single-hit multi target model [SF=1-(-e-D/D0)N] and linear-quadratic model [SF= e^(-αD-βD2)]. Results: With the treatment of different concentration of CQ on TE-1 cells for 48 hours, cell viability reduced in a dose-dependent manner and its values of IC50 and IC10 were 72.332±5.277μmol/L and 15.416±3.327μmol/L, respectively. The concentration of 15μmol/L was chosen for the following experiments. Western-blot showed the expression of