慢性乙型肝炎患者前S1蛋白C末端IgG抗体与干扰素早期疗效相关性-内科学(传染病)专业毕业论文.docx

安徽医科大学硕士学位论文 安徽医科大学硕士学位论文 6 6 12th week after the treatment. Results 21 patients were positive for anti-preS1 antibody, and 48 patients were negative for anti-preS1 antibody. The average decrease in viral levels was 3.37lg copies/ml and 0.33lg copies/ml in anti-PreS1 positive patients and anti-preS1 negative patients, respectively 12 weeks after the treatment, the difference between the two groups was significant (Z = -3.658, P = 0.000 ); the average decrease in ALT levels was 92 U/L and 30.5 U/L in two groups, respectively (Z = -2.132, P = 0.033). The rate of hepatitis B e antigen (HBeAg) loss was 41.2% (7/17) and the rate of anti-HBe seroconversion was 5.9% (1/17) in anti-PreS1 positive group, however, the rate of hepatitis B e antigen loss were only 12.8% (5/39) and none of the patients in anti-PreS1 negative group showed anti-HBe seroconversion, difference between the two groups was significant (Z = -5.110, P = 0.000). The rates of response were 71.4% (15/21) and 16.7% (8/48) , respectively in anti-PreS1 positive group and anti-PreS1 negative group. The rates of complete response were 23.8% (5/21) and 6.25% (3/48) , respectively in these two groups. The positive predictive value (PPV) of anti-C-terminal region of preS1 (94-117aa) antibody in predicting early response was 71.6% and the negative predictive value (NPV) was 83.3%. Conclusions Detection of anti-C-terminal region of preS1 (94-117aa) antibody may help to improve the efficacy of INF-alpha therapy for chronic hepatitis B (CHB). Keyword Hepatitis B, chronic/Interferon-alpha/ Early response/pre-S1 PAGE PAGE 1 中英文词对照表 英文缩写 英文全称 中文全称 HBV Hepatitis B virus 乙型肝炎病毒 DNA Deoxynucleic acid 脱氧核糖核酸 PCR Polymerase chain reaction 多聚酶链反应 EDTA Ethylene diaminetetraacetic acid 乙二胺四乙酸 EB Ethidium Bromide 溴化乙锭 TBE Tris-base, boric acid, EDTA buffer Tris碱、硼酸、EDTA缓冲液 Tris Tris(hydroxymethyl)aminomethane 三羟甲基氨基甲烷 rpm Rotate per minute 每分钟转数 SDS Sodium dodecylsulfate 十二烷基硫酸钠 ELISA Enzyme-Linked Immunosorbnent Assay 酶联免疫吸附