黄颡鱼保活技术分析-环境科学专业毕业论文.docxVIP

上海海洋大学硕士学位论文 上海海洋大学硕士学位论文 II II 研究了黄颡鱼的有水保活技术，影响有水保活时间的因素和保活过程中鱼体 肌肉、血液和水体指标变化情况。实验结果表明，黄颡鱼在 5 ℃充氧，鱼水比 1:1 条件下，保活时间最长为 102 h，保活结束后放入常温水中 10 min 可完全恢复常态。 在保活过程中肌肉中糖原含量下降，乳酸含量上升，肌肉 pH 下降；水体溶氧下降， 氨氮含量上升，pH 基本无变化；血液中肌酐、血糖、乳酸、尿酸含量在保活过程 中显著上升，白蛋白在保活中显著下降，总蛋白先下降后恢复正常水平，尿素含 量基本没有发生显著性改变。 关键词：黄颡鱼；保活；麻醉；丁香酚；生态冰温 III III Study on the keep-alive technology for yellow catfish ABSTRACT In this study, the ecological ice and anesthesia was used to research the keep-alive technology of yellow catfish, designed to explore and find out the related technical parameters for catfish transport, the most suitable conditions to determine the different keep-alive technology to maximize the benefits of transport in different environments and extend the transport time. This research examined the keep-alive transport technology without water of yellow catfish in the ecological ice-temperature zone, the impact factors, and changes of biochemical indexes in fish muscle and blood. The result showed that ecological ice-temperature zone of yellow catfish was -1~4 ℃, and the temperature, oxygen supply and temporary culture time were the main impact factors that affected the survival time of yellow catfish. After two days temporary culture, the yellow catfish could survive for 24 hours with pure oxygen at 2 ℃, and the catfish could successfully revive within about 20min when put them into water at the ambient temperature; Lactic acid in muscle increased, whereas glycogen content and pH decreased during keep-alive process, levels of creatinine, blood glucose, lactic acid in blood were significantly increased, no significant alternations were observed in the other biochemistry parameters before and after keep-alive experiment. General water bath was used to study the anesthetic effect of eugenol on yellow catfish, the aim of this paper was to determinate the anesthetic effect and the changes in respiratory frequency of different concentrations of eugenol on yellow catfish, the ef