利用组学方法研究亚洲璃眼蜱唾液腺协助其高效吸血的分子机制-生态学专业毕业论文.docxVIP

面的了解亚洲璃眼蜱唾液腺的生理机能，以及更深层次的调控机制和部分物质的 面的了解亚洲璃眼蜱唾液腺的生理机能，以及更深层次的调控机制和部分物质的 代谢规律，为研究和探寻蜱类的综合防治提供了有力的理论支撑。 关键词：亚洲璃眼蜱；转录组；定量蛋白质组；iTRAQ 万方数据 AbstractTick Abstract Tick is the second largest blood sucking arthropod in the world，and blood sucking process not only destroys the host(including human)skin causes pathogen infection，while tick is many extremely dangerous pathogens media，seems like： Borrelia burgdorferi，Anaplasma phagocytophilurn,typhus fever etc．So it is of great significance to the research．Female tick would undergo great changes especially after mating and it can increase rapidly more than ten times．The blood provides the raw materials and energy to complete the spawning activities，SO analyzing the marker molecule of different stages is meaningful for comprehensive understanding the special lifecycle of tick．Salivary gland is a very important tissue for tick，because it promot a lot of blood sucking in the short period of tick．So to explore the molecular mechanism of salivary glands become importance method that prevention and treatment oftick． We adopted the generation sequencing technology(Illumina HiSeq2500)to obtain the four stages of female tick(starvation period，unmated blood feeding period， mated half full period and full blood period)gene libraries．In order to find the specific genes expressed in different blood sucking period．At the same time，the data is used as the Database for the quantitative proteomic analysis of salivary gland．By the quality controlling，we got 1 6．39Gb Clean Data．The percentage of Q30 bases in four samples are not less than 82．9 1％．We got 7 1 586 Unigenes that owing N50=2 1 09 through asembling reads from the clean data．Than，Unigenes were putted to seven different data bases named：Nr,swissprot，COG,KOG,GO，KEGG and Pfam and performing blast and as a result，256 1 9 Unigenes were annotated functions．As a result，we got a group that eluding 1 54 1 4 annotated genes which coming from blasting of swissprot．Than we performed the GO analysis