冷却牛肉菌落总数生长模型及货架期预测模型的研究-农产品加工及贮藏工程专业论文.docxVIP

冷却牛肉菌落总数生长模型及货架期预测模型的研究 The pearson quotas between TPC and main spoilage organisms were best correlated.The result showed that it was available to model TPC in chilled beef as the pearson indexes between TPC and Pseudomonas, Acinetobacter, Lactobacillus, Carnobacterium were higher than 0.94. In some extent, TPC can reflect the quality of beef. The equation of linear regression of TPC and TVB-N was established y=4.347+0.2432x (R2=91.40% Pr=0.0029) according to the best correlation between TPC and TVB-N. The spoilage limit of TPC is 107.995cfu/g when TVB-N reached 15mg/100g. The Modified Gompertz models were set up in constant temperatures and R2 values were greater than 0.95. The square root model was used to consider the maximum specific 1/2 growth rate of TPC and temperature (μmax -t), and the secondary model of were obtained: μ1/2=0.01412(t+6.859) (R2=0.9651).The macimum specific growth rate and temperature showed good linear relationship and residual values were fluctuant by 0. The experimental datas were in 95% confidence intervals between predicted upper limit and lower limit. The Modified models were validated at constant temperatures (4℃and 10℃) and fluctuant temperatures. The results showed that the predicted values fluctuated in a range of about 10%, and the differences between predicted and observed values (Af) were less than 20%. So the predict models of TPC can predict the microbial quality of chilled beef during storage. The predictive model of shelf life was constructed as follows: SL=?－[(8.346－ N0)/μmax×2.718]×{ln [-ln[(7.995－N0)/(8.346－N0)]]－1}. The shelf life can be predicted from the equation if N0 and temperature T are determined. Key words: chilled beef, TPC, Predictive Model, shelf life 4 符 号 说 明 HACCP: Hazard Analysis Critical Control Point, 危害分析与关键控制点 CCP: Crical Contol Point, 危害控制点 TVB-N: Total volatile basic nitrogen, 挥发性盐基氮 TBA: Thiobarbituric acid assay, 硫巴比妥酸 TMA-N: Trimethylamine nitrogen, 三甲胺氮 cfu: Colony forming units, 菌落形成单位