桑天牛长尾啮小蜂寄主定向的挥发性信息物质研究-林木遗传育种专业论文.docxVIP

Research Research on the volatile infochemicals for the host location ofAprostocetusfkutai,an egg parasitoid of apriona germari Author：yang yuan Supervisor：lijiquan Major：Forest heredity and breeding Abstract Aprostocetusfukutai is all egg parasitoid ofApriona germari．wj designed three steps to find the infochemicals for the host location of the wasp．First of a11．we devised several treatement that maybe include the volatiles in the process，and determine the behavior resporise of the wasp to each treatment by y-olfactometer,thell we collected and indentified the volatiles of intact mulberry shoot，host feeding damaged mulberry shoot， and host oviposited mulberry shoot．Several compounds that are likely to be the allure matedals are choosed by comparing differences of volatiles from different treatment of shoots in quality and quantity．Finally three compounds are confirmed in the host location of the wasp through the farther study of EAG and behavior determination．The concrete results ofthe study are as follows： 1．T11e healthy plant(HP)and the system damaged(SDl plant are all attractive to the wasp significantly；the mechanical wounding plant(MW)and the feeding damaged plant(FD)attracted the wasps more clearly；the wasps strongly tend to the integer plant and the oviposited plant(oP)．There are no significant differences between the HP and the MW； compared with the MW,FD are nlore attractive to the wasps．But the wasps tend to the OP much easier than the FD．The volatiles from female and male host insectsApriona germaH are not attractive to the wasps，but the frass ofthe host is attractive to the wasp． 2．44 compounds are analysized by TcI：GC／MS，mainly include ah lehydes， ketones，aromatic compounds，terpenoids and nitrogenous compoun ntent of terpenoids in the volatiles are high,and becomes the maximum in volat nents of feeding damaged plants． There are 27 compounds in the volatiles of the host frass，which consists of hydrocarbons，alcohols，aldehydes，ke