少阳生骨方对SD大鼠体外软骨细胞培养及在体软骨损伤影响的实验分析-中医外科学专业论文.docx

成都中 成都中医药大学博士学位论文 PAGE PAGE 5 2．不同浓度少阳生骨方含药血清，均能提高SD大鼠体外第五代软骨细胞II 型胶原及蛋白多糖表达及合成，维持软骨细胞表型稳定，但作用有限。 3. 少阳生骨方能促进SD大鼠在体软骨损伤后软骨的修复，且修复组织更接 近透明软骨。 4. 少阳生骨方能明显降低SD大鼠在体软骨损伤后关节液的IL-1 β水平，从 而促进软骨细胞的修复。 关键词：少阳生骨方；关节软骨；损伤；去分化；实验研究；软骨细胞。 Experimental study of ShaoYangShengGuFang Prescription on the SD rat articular chondrocyte in vitro culture and cartilage injury in vivo Abstract objective: Articular cartilage injury has become a common orthopaedic clinical disease, but its ability to self-repair is extremely limited. and so has been the difficulty in joint surgery field and research focus.This subject based on the theory of shaoyang dominating boneto apply ShaoYangShengGuFang Prescription intervening with the rat articular chondrocyte in vitro culture and animal models of cartilage injury in vivo.By CCK-8 method to detect cell proliferation,Immunohistochemical method to detect Ⅱtype collagen,RT - PCR method to detect Ⅱ type collagen mRNA expression, radiation immunity analysis (RIA) determination of cytokine changes of IL - 1β.And the change of articular cartilage tissue morphological and ultrastructure,at the level of cell and molecular, to explore the the feasibility and the possible mechanism of the treatment of cartilage damage and provide strong experimental evidence for the application of Chinese medicine theory ,open new avenues for the treatment of cartilage injury. Laying the foundation for the next step to continue to study the mechanism of the Prescription repair cartilage damage and elucidate the role of the Prescription from PAGE PAGE 7 the the molecular Biology and Cytology level , provide strong evidence for clinical promotion. Methods :this experiment was divided into four parts. Part Ⅰ: Efects of ShaoYangShengGuFang Prescription containing serum on the articular chondrocyte of SD rat in vitro culture chondrocytes were obtained and cultured in vitro from 1 weeks SD rat.The third passages were seeded in culture flask according to the density of 1 * 105 / m