离子液体水两相体系中酵母细胞催化的2’氯苯乙酮选择性还原-生物化工专业论文.docxVIP

浙江大学颁。h学位论文Abstract 浙江大学颁。h学位论文 Abstract With the progress in pharmacology of chiral pharmaceuticals and the strict regulation in the application of new drug in recent years，the research and development of chiral pharmaceuticals has become the new direction internationally．Many researchers are applying themselves to develop new processes for chiral compound synthesis谢m 11igh conversion ratio and hirgh stereo—selectivity，in which，bioWansfonnation is attracted interests because ofmild reaction condition and hi曲stereo-selectivity Ionic liquids(ILs)have been vigorously investigated as possible replacements for volatile organic solvents．This work presents the use of imidazolium Ionic Liquids([C。mim][PF6])in biphasic system in the asymmetric reduction of 2-chloro-acetophenone(2’一CI-AP)to(S)一1-(2一chloro-phenyl)ethanol((S)一1-2一 C1-PE)catalyzed by Candida Pseudotropicalis A．The biocompatibility of imidazolium ionic liquids is carefully assayed before using．The results suggest that an increase in alkyl group chain substituted on the cation ring correspond with an increase in toxicity．It is also found that the ratio of ILs in the biphasic system influence the performance of the reaction distinctly Under the optimal conditions，the maximum yield and product e．e．were 85％ and99．9％．Additionally,immobilized yeast cells exhibits greater biocompatibility with ionic liquids，and could be used repeatedly in biphasic system in whole cell biocatalytic process． Keywords：Ionic Liquid；Biocatalysis；Asymmetric reduction： 浙江大学硕士学位论文—1上．—jL—- 浙江大学硕士学位论文 —1上．—jL—- 日}『舌 离子液体是一种目前广泛研究的新型绿色溶剂，由于其独特的性质，有望取代 传统的挥发性有机溶剂。利用生物催化和转化合成手性药物及其中间体是国际新药 研究的热点。本论文的主要工作就是将二者结合起来应用于不对称合成，研究在眯 唑类六氟磷酸盐离子液体中利用酵母细胞催化的不对称还原反应选择性还原2’一氯一 苯乙酮制备重要手性药物中间体(S)-2’一氯一苯乙醇。 2’一氯一苯乙酮是一种疏水性底物，在水中的溶解度比较低(O．049／L)。而在本 实验所构建的离子液体／水两相体系中，由于离子液体对底物良好的溶解性，因此可 大大提高底物在反应体系中的浓度(39／L)，这是本反应的一大特点。 在本课题的研究中，考虑到离子液体对实验中选用的生物催化剂一酵母，可能 会存在一定的毒性，因此，详细考察了咪唑类离子液体对酵母细胞活性的影响。同 时分析了离子液体／水两相体系中影响反应的各种影响因素，并尝试了将固定化酵母 应用于离子液体／水两相体系中还原反应。 浙江尺学硕士学位论文第一章文献