拟缺香茶菜提取物生物活性的研究.docVIP

拟缺香茶菜提取物生物活性的研究 陈慧平，马方1,王瑞娟2,邹敏1,师孟超1,罗玉1,郭裁1 (1、河南省医药科学研究院，河南郑州450052； 2、郑州大学第一附属 医院，河南郑州450052) 目的研究拟缺香茶菜乙醯和乙酸乙酯提取物的体外抗肿瘤及抗菌活性。方 法采用MTT法测定拟缺香茶菜乙瞇和乙酸乙酯提取物对人肝癌细胞株HepG2 增值的抑制活性，用试管稀释法测定拟缺香茶菜乙瞇和乙酸乙酯提取物对金黄色 葡萄球菌、大肠艾希菌等菌种的抗菌活性。结果拟缺香茶菜乙瞇提取物对人肝 癌肿瘤细胞HepG2具有一定的抑制作用，并在测定浓度范围内呈现良好的剂量 依赖性，月?对金黄色葡萄球菌和大肠艾希菌这两种菌株有较灵敏的抗菌活性。结 论拟缺香茶菜乙醯提取物具有较显著的体外抗肿瘤及杀菌活性，为今后分离出 抗癌及抗菌单体化合物提供科学的参考依据。 关键词拟缺香茶菜；抗肿瘤；抗菌活性 Study on the biological activity of the Isodon excisoides extracts CHEN Huipingl, MA Fangl, WANG Ruijuan2? ZOU Mini, SHI Mengchaol,LUO Yul,GUO Weil (1. Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052, China ； 2.The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China) Abstract Objective: To study the antitumor and antibacterial activity of Isodon excisoides extracted by dieth-yl ether and ethyl acetate. Methods: Using diethyl ether and ethyl acetate ethanol as the extracted solvents, the vitro cytotoxicity study was con?ducted based on the above kinds of extracts by MTT method;To study the antibacterial activity of Isodon excisoides extracted by dieth-yl ether and ethyl acetate, the tube dilution method was uaed for determination of Isodon excisoides extration ? Results: In the wake of the different concentrations of isodon excisoides extracts, the proliferation inhibition rate of the kinds of tumor cell is different. There are more sensitive antimicrobial activity against Staphylococcus aureus and Escherichia coli.Conclusion: Isodon excisoides ether extract show better antitumor and antibacterial effects, this provided a scientific justification for the future separation of anticancer and antibacterial compounds. Keywords: Isondon excisoides; antitumor; antibacterial activity 拟缺香茶菜是唇形科香茶菜属植物，资源丰富，种类繁多，广泛分布于亚洲 和非洲各地，在我国约有90余种，除新疆、青海及内蒙古外，几乎遍布全国。 河南省自然科学基金研究项目资助142300410230 河南省自然科学基金研究项目资助152300410160 该属植物具有清热解毒、活血化瘀、抗菌消炎、抗肿瘤、治疗各种肝炎等功 效， 民间常作为清火、抗癌、消炎和抗菌药应用 1H2]o本实验研究发现拟缺 香茶菜 乙醴提取物对肿瘤细胞有较强的抑制作用，此外还有抗菌活性，进一步确定 了拟缺香茶菜乙酬提取物为抗肿瘤及抗菌