探讨蛋白酶体功能障碍对散发性帕金森病发病机制的影响.docVIP

探讨蛋白酶体功能障碍对散发性帕金森病发病机制的影响 (广东三九脑科医院神经内一科 广东 广州510510) 摘 要 目的探讨蛋白酶体功能障碍对散发性帕金森疾病的发病机制研 究。方法将蛋白酶抑制剂注入大鼠的左侧黑质致密部位，构建蛋白酶体功能障 碍模型作为观察组，对照组采用牛理盐水注射，观察两组大鼠的行为学改变情况, 通过HE染色检测路易(小)体形成率，RT-PCR Western blotting分子牛物学方 法检测黑质部位alpha;-突触核蛋白(alpha;-Syn)表达情况。结果HE染色结 果均显示路易(小)体形成率观察组高于对照组，两组间相比具有统计学差异(P 0.05)； RT-PCR、Western blotting分子牛物学检测结果显示alpha;-突触核蛋白 观察组显箸高于对照组，两组间相比具有统计学差异(P0.05)o结论蛋白酶 体功能障碍可以导致路易(小)体形成率及黑质部位alpha;-突触核蛋白 (alpha;-Syn)形成是散发性帕金森疾病的危险因素。 关键词 路易(小)体形成率；alpha;■突触核蛋白(alpha;-Syn)；散发性帕 金森；发病机制 R742 A 1004-6194 (2015) 02-0330-02 To investigate the impact of proteasome dysfunction in sporadic ParkinsorVs disease pathogenesis [Abstract ]Objective: To investigate the proteasome dysfunction in the study of the pathogenesis of sporadic Parkinsons disease. Methods: Protease inhibitor injection site in substantia nigra of rat left the building proteasome dysfunction model as the observation group, the control group with saline injection, observe the behavior of the two groups of rats changes the situation, by HE staining Louis (small) format!on rate, RT-PCR, Western blotting molecular biology methods to detect the substantia nigra alpha;- synuclein (alpha;-Syn) express!on. Results: HE staining showed Louis (small) format!on rate were higher, compared with the significant difference between the two groups (P lt;0.05); RT-PCR, Western blotting molecular biology test results show that alpha;- synuclein observation group were significantly higher compared with the sign 讦 icant d iff ere nee betwee n the two groups (P lt;0.05). Conclusion: The proteasome dysfunction can lead Louis (small) formation rate and the substantia nigra alpha;- synuclein (alpha;-Syn) is a risk factor for sporadic form of Parkinsons disease. Keywords］ Louis (small) formation rate; alpha;- synuclein (alpha;-Syn); sporadic Parkinson; Pathogenesis 原发性帕金森是一种慢性的神经系统疾病，是i种中老年的常见性疾病, 临床上主要表现为震颤、肌肉强直、运动迟缓等，主要的病理特征是出现黑质的 致密部位多巴胺能神经元的选择性丧失，出现残存路易小体，该病包括帕金森病 痴呆和路易体痴呆，其中90%的患者呈现散在发生，即散发性的帕金森⑴。目前 该病主要的治疗方法包括药