氢醌对L02肝细胞损伤的研究.docVIP

摘要：［目的］探讨氢醍(HQ)对L-02肝细 胞损伤及其可能的作用机制。［方法］应用曝卩坐蓝 (MTT)比色法检测浓度分别为0、5、10、20、40、 80、160 和 320 U mol/L 的 HQ 作用 24 h 后对 L-02 肝 细胞存活率的影响；利用万能倒置显微镜观察不同浓 度HQ染毒之后L-02肝细胞的形态学改变并摄影成像; 利用全自动生化分析仪检测不同浓度HQ染毒24 h后 L-02肝细胞的乳酸脱氢酶(LDH)漏出率和丙氨酸氨 基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性， 以观察HQ对L-02肝细胞膜完整性的影响。［结果］ 在0?80 U mol/L浓度的范围内染毒24 h后，HQ对 L-02肝细胞的存活率没有明显的影响(P0.05)；浓 度2160 ? mol/L时,染毒24 h后，存活率则明显下 降(P 关键词：氢醍；L-02肝细胞；细胞毒性 A Study of the Damage of Hydroquin one on L-02 Hepatic Cells HU Gong-hual, 3，ZHUANG Zhi-xiong2*, YANG Ling-qing2, SHA Yan3, YANG Jian-ping3 (l.Department of Preventive Medicine，Gannan Medical College, Ganzhou 341000, China； 2.Shenzhen Center for Disease Dontrol and Prevention，Shenzhen 518020, China； 3.Department of Preventive Medicine, School of Public Health, SUN YAT-SEN University, Guangzhou 510080, China) Abstract： [Objective] To characterize the genereal toxic effects of hydroquinone (HQ) to L-02 hepatic cells and its possible mechanisiri. [Methods] MTT assay was con ducted to detect the effects of HQ in various concentrations (0, 5，10, 20，40，80, 160, and 320 u mol/L) for 24 hrs on the viability of L-02 hepatic cells? Morphological change of such treated L-02 hepatic cells was also observed by versatile in verted microscope and captured ? Fin ally, the leakage rate of LDH and the activities of ALT and AST in the cultured medium of hepatic L-02 cells treated with HQ were measured by Automatic Biochemistry Analyser. [ Results] MTT assay showed that the survival rate of L-02 had little effect from the treatment with HQ in the concentration from 0 to 80 u mol/L( P 0.05)，whereas the survival rate in the groups of 160 u mol/L and 320 P mol/L after treated with HQ for 24 hours was significantly lower than that in the control （P 1.1.2主要仪器HFsafe-1200型生物 安全柜、HF212UV型CO2恒温培养箱（均为上海力申 科学仪器公司），XDS-1B型倒置生物显微镜（重庆光 学仪器厂），TDL-5-A型低速离心机（上海安亭科学仪 器厂），BP1211S型电子天平（德国赛多利斯有限公司）, Milli-Q Biocel型超纯水系统装置（法国MILIPORE公司）, HVE-50高压灭菌器（日本HIRAYAMA公司），SUT-6120 热空气消毒箱（德国Heraeus公司），MM-I型微量振 荡器（上海精科实业有限公司），Power