离子液体提取分离光甘草定的研究应用化学专业论文.docxVIP

II II Abstract Glabridin is the main hydrophobic ingredient in flavonoids. The glabridin content is about 11% in licorice flavonoids and about 0.2% in Glycyrrhiza glabra L.. Hence, the separation and purification technologies of glabridin are the key processes in the development of Glycyrrhiza glabra L.. In this pater, the extraction of glabridin from Glycyrrhiza glabra L, the extraction and mechanism of glabridin using ionic liquids were studied. The research of glabridin are as follows: A reverse phase high performance liquid chromatographic method is used for determination of glabridin in Glycyrrhiza glabra L.. The yield of glabridin is regarded as evaluation index. The effects of extraction solvent, extraction method and extraction technology are studied. The results show that the microwave-assisted extraction method is appropriate for extraction of glabridin. The optimal extraction technology is extraction solvent of ethanol/water (70:30, V/V), the solid/liquid ratio of 1:15 (g/mL), extraction temperature of 65 oC, extraction time of 40 minutes and times of one. The yield of glabridin reached to 2.56‰. To extract glabridin from licorice extract using imidazolium-based ionic liquids (ILs), a series of 1-alkyl-3-methylimidazolium ionic liquids with different anions and alkyl chain lengths of cations are selected. The different ionic liquids, extraction technology and back extraction of ionic liquid are investigated. It is concluded that the optimal IL is 1-Hexyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([C6mim][NTf2]). The extraction efficiency of glabridin using [C6mim][NTf2] is 96.04% under the optimal conditions of 0.40 mg·mL?1 glabridin concentration, pH = 7, phase volume ratio (VIL/VLE) of 1:3, and extraction time of 40 min. The back extraction of glabridin from [C6mim][NTf2] is used by 2.0 mol·L?1 sodium hydroxide solution. The recovery rate of glabridin is 98.96% in the first cycle, ionic liquids may recycle 5 times at least. D