不同植物无色花青素还原酶及其基因的生物信息学分析.docxVIP

不同植物无色花青素还原酶及其基因的 生物信息学分析 李蒙鲍牢 西安文理学院生物与环境工程学院 摘要： 对9种不同植物的无色花青素还原酶及其基因进行生物信息学分析，探索该蛋 口的结构并预测LAR的功能。通过生物信息学软件对Gen Bank中已经登录的不 同植物LAR基因和氨基酸序列进行分析，对其组成成分、理化性质、翻译后修饰 位点、功能域、二级结构、亚细胞定位、分子进化等进行预测和推断。结果表明, 9种植物无色花青素还原酶基因全长在l?0l.2kb,编码34291个氨基酸，不具 有信号肽;9种植物LAR都具有蛋片激酶C磷酸化位点、酪蛋白激酶II磷酸化位 点和N-端肉豆蔻酰化位点，个别植物LAR具有N-端糖基化位点、酪氨酸激酶磷 酸化位点和c AMP和c GMP依赖蛋白激酶磷酸化位点;二级结构以a螺旋和无规 则卷曲为主;进化分析表明禾木科的玉米与其他科植物亲缘关系较远。 关键词： 无色花青素还原酶;生物信息学;结构预测;功能分析; 作者简介：李蒙（1988—）,男，助理实验师，硕士，研究方向为生物化学与分 子生物学。 收稿日期:2017-05-14 Bioinformatics Analysis of Leucoanthocyanidin Reductase and Gene in Different Plants LI Meng BAO Feng College ofBiological andEnvironmental Engineering, Xian University of Arts and Sciences; Abstract： The leucoanthocyanidin reductase (LAR) and its gene in nine kinds of plants were analyzed by using bioinformatic method. The nuoleic acid scqucncos and amino acid sequences of LARs registcred in Gen Bank were analyzed by using bioinformatic software, and their compositions, physical and chemical properties, post-translational modification sits, functional domains, secondary structure, subcellular location and molecular phylogenetic evolution were predicted and inferred. The resuIts demonstrated that the LAR genes in nine kinds of plants was 1. 0 1. 2 kb in overall length, and they coded 342 ?391 amino acids, but had no signal peptide. All LARs had protein kinase C phosphorylation sites, casein kinase II phosphorylation sites and N-myristoyla.tion sites, and the LAR in several plants al so had N-glycosylation sites, tyrosine kinase phosphor^dation sitcs, and c AMP-dependent and c GMP-dcpcndcnt protcin kinase phosphorylation sites. The secondary structure mainly was of alpha helix and random coil. The evolutionary tree showed that the gramineous maize and the pla nts in other families had a distant gen etic relati on ship. Keyword： Lcucoanthocyanidin reductase; Bioinfooiatics; Structureil prcdiction; Functional analysis; Received： 2017-05-14 无色花青素还原酶(Leucoanthocyanidin reductase, LAR, EC 1. 17. 1. 3