PolyIC作用于猪只抗原呈献细胞TLR基因表现之探讨.docVIP

Poly I:C作用於豬隻抗原呈獻細胞TLR基因表現之探討 Stimulation of Poly I:C on TLR Gene Expressions in Porcine Antigen-Presenting Cells 研究生：陳乾瑋 Chen, Chein-Wei 指導教授：莊秀琪Chaung, Hso-Chi 【摘要】 抗原呈獻細胞以病原相關分子型態辨識受體 (pattern recognition receptor；PRR)偵測外來之病原相關分子結構(pathogen associated molecular pattern；PAMP)，而誘發細胞內之訊息傳遞作用，活化細胞內特定細胞激素基因表現，進而調控後續之免疫反應。PRR包括類鐸接受體（Toll-like receptor; TLR）以及鈣依賴性凝集素受體（C-type lectin receptor），近年來已證實人至少有13種不同的TLR，但有關豬之TLR研究則十分闕如。本實驗目的即探討poly I:C對豬隻肺泡巨噬細胞 (alveolar macrophage；AM)、骨髓造血母細胞衍生之未成熟樹狀突細胞 (immature dendritic cells; imDC) 及成熟樹狀突細胞 (mDC) TLR基因表現之影響。結果顯示AM之TLR3與8高於imDC與mDC，mDC之TLRs 基因表現則均未有高於AM與imDC者。此外，poly I:C的刺激增加AM之TLR3，但抑制AM之TLR7與TLR8以及imDC之TLR8基因表現，在poly I:C刺激下，imDC之TLR9基因表現則顯著高於AM和mDC。 關鍵字：TLR3、Poly I:C、AM、imDC、mDC 【Abstract】 Pattern recognition receptors (PRR) include two superfamilies, Toll-like receptors (TLR) and C-type lectin receptors. The expressions of PRRs on immune cells function to recognize different pathogen associated molecular patterns (PAMP) and thus induce different signal pathways in cell, which in turn causes the secretion of cytokines to regulate the acquired immune responses. Thirteen different TLRs have been demonstrated in human, however, there are only a few literatures on porcine TLRs. In this study, the effects of poly I:C on TLRs expressions of three porcine antigen-presenting cells including alveolar macrophage (AM), immature bone marrow haematopoietic-derived dendritic cells (BMHC-imDC), mature DC (BMHC-mDC) were investigated. The results showed that the TLR 3 and 8 gene expressions in AM were higher than those in imDC and mDC. No significantly higher expressions of TLRs were observed in mDC. Furthermore, the stimulation of poly I:C may inhibit the gene expressions of TLR 7 and 8 in AM and TLR8 in imDC but enhance the expression of TLR3 in AM. Under the stimulation of poly I:C, the expression of TLR9 in imDC was the highest among these three antigen-presenting cells. Keywords: TLR3, Poly I:C, alveolar macrophage, dendrit