提高局部ALA透皮吸收和生物利用度的研究.docVIP

提高局部ALA透皮吸收和生物利用度的研究 佘文莉1 陆红21暨南大学附属第一医院整形美容激光科 广东 广州 510632 2 暨南大学附属第一医院急诊科 广东 广州 510632 【摘要】目的：探讨提高局部ALA透皮吸收和生物利用度的方法。方法：建立迷你家猪动物模型，分别采取不同孵育温度、溶剂、pH值、有无角质软化剂等孵育方法进行孵育，测定不同孵育方法孵育后病变组织光敏剂的荧光强度，观察不同孵育温度、溶剂、pH值、有无角质软化剂等孵育方法对ALA透皮吸收度和生物利用度的影响。结果：42度条件下孵育组的病变组织中光敏剂的荧光强度显著高于在37度条件下孵育组的荧光强度（P0.05）；使用月桂氮卓酮配置的ALA溶液孵育组病变组织中光敏剂的荧光强度均明显高于在生理盐水配置的ALA溶液条件下孵育组（P0.05）；在pH 4.0ALA溶液条件下孵育组病变组织中光敏剂的荧光强度均明显高于在pH 7.0和pH10.0的ALA溶液条件下的孵育组（P0.05）；使用角质软化剂预敷组的病变组织中光敏剂的荧光强度均明显高于未经过预敷组（P0.05）。结论：较高的孵育温度、较低的pH值以及透皮促进剂和角质软化剂的加入均有利于提高局部ALA透皮吸收和生物利用度。 【关键词】ALA；透皮吸收；生物利用度； Study on How to Improve?the?Local?ALA?Transdermal Absorption?and Bioavailability Shewen-li1 luhong2 1 First Affiliated Hospital of Jinan University, cosmetic laser Guangzhou 510632 2 Emergency department of the First Affiliated Hospital of Jinan University, Guangzhou 510632, China [Abstract] Objective: To dicuss how to improve?the?local?ALA?transdermal absorption?and bioavailability. Methods: After the construction of mini-pigs model, the lesions were incubated by different temperatures, solvents, pH values and horny?softeners. The influence on ALA?transdermal absorption?and bioavailability with different temperatures, solvents, pH values and horny?softeners were observed. Results: The photosensitizer’s?fluorescence intensity of?the?lesions in the group incubated by 42℃ was stronger than that in the group incubated by 37℃ (P0.05). The photosensitizer’s?fluorescence intensity of?the?lesions in the group incubated by Azone was stronger than that in the group incubated by saline (P0.05). The photosensitizer’s?fluorescence intensity of?the?lesions in the group incubated by pH4.0 was stronger than that in the group incubated by pH7.0 and pH10.0 (P0.05). The photosensitizer’s?fluorescence intensity of?the?lesions in the group incubated by horny?softener was stronger than that in the group incubated without horny?softener (P0.05). Conclusion: The higher incubation temperature, lower pH and the use of penetration enhance