胃转流术降低2型糖尿病大鼠肝脏FoxO1因子的表达.docVIP

PAGE PAGE 10 作者简介：陈志雄（1985-），男，硕士研究生在读，主要研究方向胃肠道内分泌外科 通信作者：张 军（1965-），男，副教授，主要研究方向2型糖尿病的外科治疗及胃、结直肠癌的发病机制。Email zjun2323@ 胃转流术降低2型糖尿病大鼠肝脏FoxO1因子的表达 陈志雄 张 军* 张刘平 寇耀 蒙长远 张能 汪志红 （重庆医科大学附属第一医院胃肠外科 重庆400016） [摘要] 目的 研究胃转流术对2型糖尿病大鼠肝脏FoxO1表达的影响,初步探讨胃转流术改善2型糖尿病的机理。方法 40只SD大鼠随机分为糖尿病手术组（A组）、假手术组（B组）、正常对照组（C组）、糖尿病对照组（D组）。C、D组按A、B组术前和术后的4、8、16、24周检测空腹血糖、空腹和餐后血清胰岛素(Insulin)、胰高血糖素样肽（GLP-1）。24周后处死各组大鼠，免疫组化和western blot检测各组肝脏中FoxO1的表达。结果 A组术后4周空腹血糖由术前（20.2±2.1）mmol/L降至（10.5±2.5）mmol/L；术后24周空腹血糖为（7.7±1.1）mmol/L。术后4周空腹Insulin由术前（2.87±0.08）mU/L增至（2.91±0.08）mU/L，术后24周空腹Insulin为（3.0±0.06）mU/L；术后4周空腹血清GLP-1由术前（8.8±0.5）pmol/L升至（11.9±1.4）pmol/L，术后24周为（20.5±2.5）pmol/L；A组术后24周肝脏FoxO1表达量为18.4±1.2；B组为35.8±0.4；C、D两组分别为9.2±1.0、37.6±0.5，A组术后FoxO1的表达量明显低于B、D组，差异有统计学意义（p＜0.05）。A组血清GLP-1浓度与肝脏FoxO1的表达量成负相关（r=-0.864,p＜0.05）。结论 胃转流术可显著降低2型糖尿病大鼠肝脏FoxO1的表达，降低血糖。 关键词：胃转流术；2型糖尿病；FoxO1 Effects of Gastric Bypass on the expression of FoxO1 in type 2 diabetes rats’liver Chen Zhixiong, Zhang Jun*,Zhang Liuping, Kou Yao,Meng Changyuan, Zhang Neng , Wang Zhihong （Department of Gastrointestinal Surgery, the First Affiliated Hospital of Chongqing Medical University , Chongqing 400016, China） Corresponding author：Zhang Jun, Abstract Objective To investigate the effect of FoxO1 expression of the gastric bypass on diabetes rats’ liver. We preliminary, explore what’s the mechanism for the gastric bypass operation to ameliorate the type diabetes. Methods 40 SD rats were randomly divided into diabetes operation group (group A, n=10 ),diabetes sham operation group ( group B, n=10 ), the normal control group ( group C, n=10 ), diabetes group ( group D, n=10 ).The levels of fasting, postprandial, insulin and glucagon-like peptide-1(GLP-1) were measured and compared among the A and B groups before the operation and at 4,8,16,24 weeks following the operation , those items were also measured in C and D group. After 6 months, all rats were decapitated to retrieve livers and surveyed the expression of the FoxO1 by usin