番茄抗叶霉病基因cf-19的精细定位及抗病应好答基因筛选.docxVIP

目 目 录 3．6．2农杆菌侵染体系的建立 ．．58 4{寸论 ．59 4．1 cf-t9基因的遗传规律 59 4．2 cf-19基因介导的过敏性坏死特点 ．60 4．2．1 cf-4、cf9和缪J9基因介导的过敏性坏死特征对比分析 60 4．2．2 cfq9基因介导的抗病应答过程中关键时间点的确定 ．．60 4．3 cf-19基因的候选基因特征分析 61 4．3．1候选基因Solyc019006550．2．1为cf-4／9基因位点的Hcr9s新成员 ．6l 4．3．2候选基因与其它盯基因的进化关系分析 ．62 4．4分子标记P7可应用于分子标记辅助选择育种 62 4．5父、母本重测序与SLAF．seq结合使用可以提高定位效率 62 4．6 c／-19基因介导的抗性应答反应中的重要差异表达基因 。63 4．7 VIGS侵染体系 ．．64 5结论 ．64 6创新点 65 致谢 ．66 参考文献 67 攻读博士学位期间发表的学术论文 75 万方数据 CONTENTSCoNTENTS CONTENTS CoNTENTS Abstract in Chinese I Abstract in English．．． ．．．．．．． ．．．．． ．．． ．． ．． ．．． ．．．． ．．．．． ．．． ．．． III 1．IⅡtroduction ．．1 1．1 Advances oftomato leafmould and resistance genes ．． ． ．． ．．． ． ． ．1 1．1．1 Characteristics oftomato leafmold disease 1 1．1．2 Cladosporium／hlvum pathogen 2 1．1．3 Advances in tomato leafmould resistant genes．． ． ．．．．． ．．．．．． ．．．．．．．． ．．．3 1．1．4 Advances in tomato．C励lvum interaction 一5 1．2 Genome resequeneing technology and its application．． ． ．．．．．．． ． ．． ．．．6 1．2．1 High-throughput sequencing technology ．6 1．2．2 Application ofhigh—throughput sequencing technology ．．．．．． ．．．．． ．．．． ． ．．7 1．3 SLAF—seq technology and its application． ． ．．．． ． ．．． ． ．． ．． ．8 1．4 eDNA—AFLP technology and its application ． ． ．． ． ．．．． ．． ．．．．． ．．．．．9 1．4．1 eDNA-AFLP technology．．． ．．．． ．．． ．．．．．． ．．．． ．．．．． ． ．．．． ．9 1．4．2 Application of eDNA·AFLP technology 1 1 1．5 Objectives and significance．．．．．．． ．．．．． ． ．．．．．． ．．．． ．．．．． ．．．．．．． ．． ．．．．1 1 1．6 Main content and technique route．．．． ．．． ．．． ．． ．．．．． ． ．．．． ．．．． ．．．12 1．6．1 Main content ．．13 1．6．2’I’echnical route 一14 2．Materials and methods ．14 2．1 Resistanceidentification ofCf-199ene ． ．．． ．．．． ．． ． ．．． ． ． ． 15 2．1．1 Test materials ．15 2．1．2 Test methods ．．1 5 2．2 Study the resistance characteristics of cf-19 gene．．．．． ．．．．．． ．．． ．．．．．．．．．． ．．． ． ．15 2．2．1 Test materials．．．．． ． ． ．． ．．．． ． ．．． ．．． ．．．．． ．． ．16 2．2．2 Test methods． ．．．． ．．．．． ． ． ．．． ．． ．． ．17 2．3 Genetic analysis of cf-1